Study on the Identification of Samples Mistyped as O Phenotype and the Strategy of Safe Blood Transfusion.
10.19746/j.cnki.issn.1009-2137.2021.03.041
- Author:
Chen-Chen FENG
1
;
Jian-Yu XIAO
1
;
Li-Li SHI
1
;
Ruo-Yang ZHANG
1
;
Min XUE
1
;
Yan-Jun ZHOU
1
;
Cheng-Yin HUANG
1
;
Qing CHEN
2
Author Information
1. Jiangsu Province Blood Center, Nanjing Medical University, Nanjing 210042, Jiangsu Province, China.
2. Jiangsu Province Blood Center, Nanjing Medical University, Nanjing 210042, Jiangsu Province, China,Center for Global Health, Nanjing Medical University, Nanjing 210042, Jiangsu Province, China,E-mail：chenqing90@yahoo.com.
- Publication Type:Journal Article
- MeSH:
ABO Blood-Group System;
Alleles;
Blood Transfusion;
Fucosyltransferases/genetics*;
Genotype;
Humans;
Phenotype
- From:
Journal of Experimental Hematology
2021;29(3):910-916
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the indentification method of samples mistyped as O phenotype and to explore the precision transfusion strategy.
METHODS:The blood samples from donors and patients admitted in our center from 2018 to 2019 was collected. The samples with O phenotype suspected subtypes were further determined by tube test, adsorption-elution test, etc. Molecular testing was used to sequence the related blood type genes of the subjects.
RESULTS:Among 14 subjects misjudged as O, 11 different genotypes were identified, in which 3 blood donors were Ael02/O02, Bel03/O02, and one para-Bombay with B101/O02 (FUT1: h3h3; FUT2: Se
CONCLUSION:The phenotypes of Ael, Bel, Aw and para-Bombay subtypes are easily misjudged as type O. Molecular technology is helpful to identify the genotype of subtypes, and the corresponding transfusion strategies could be reasonably performed.
