RNA Binding Protein RBM47 Inhibits the K562 Cell Proliferation by Regulating HMGA2 mRNA Expression.
10.19746/j.cnki.issn.1009-2137.2021.03.008
- Author:
Ting LEI
1
;
Jie CUI
2
;
Jun-Ling HOU
3
Author Information
1. Department of Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University，Beijing 100038, China.
2. Institute of Medicinal Plant Development，Peking Union Medical College，Chinese Academy of Medical Sciences, Beijing 100193, China,E-mail: jcui@implad.ac.cn.
3. Beijing University of Chinese Medicine, Beijing 100029, China,E-mail: mshjl@126.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Proliferation;
HMGA2 Protein/genetics*;
Humans;
K562 Cells;
RNA, Messenger/genetics*;
RNA-Binding Proteins/genetics*
- From:
Journal of Experimental Hematology
2021;29(3):703-708
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the regulatory effects of RBM47 on HMGA2 and the function of RBM47 in human chronic myeloid leukemia cell K562.
METHODS:K562 cells were transduction by the overexpressed and knockdown RBM47 lentiviral vector. CCK-8 assay was used to detect the effect of RBM47 on the proliferation of K562 cells. Flow cytometry assay was used to detect the effect of RBM47 on the cell cycle progression of K562 cells. RNA immunoprecipitation assay was used to detect the association between RBM47 and HMGA2 mRNA. RT-qPCR was used to detect the effects of RBM47 on the stability of HMGA2 mRNA. Western blot was used to evaluate the effect of RBM47 on HMGA2 protein expression.
RESULTS:The overexpressed RBM47 could inhibit the proliferation and cell cycle progression of K562 cells. However, the inhibitation of RBM47 could improve the proliferation and cell cycle progression of K562 cells. RBM47 combined with HMGA2 mRNA could promote the degradation of HMGA2 mRNA. Thus, the overexpressed RBM47 could decrease the expression of HMGA2 protein in K562 cells.
CONCLUSION:RNA binding protein RBM47 can inhibit the proliferation of K562 cells by regulating HMGA2 expression.
