Apoptosis of Endothelial Cells Induced by Anti-Platelet Integrin β3 Antibody.
10.19746/j.cnki.issn.1009-2137.2021.02.041
- Author:
Jian-Yu WANG
1
;
Ming-Jing WANG
2
;
Ping SUN
3
;
Yan SUN
2
;
Xue-Zhe WANG
4
;
Xiao-Mei HU
2
;
Ri-Cheng QUAN
5
;
Simon-Xun LIANG
6
Author Information
1. Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121000, Liaoning province, China.
2. Department of Hematology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
3. Department of Hematology, Jining First People's Hospital, Jining 272000, Shandong Province, China.
4. Department of Clinical Laboratory, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001，Liaoning province, China.
5. Department of Hematology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China,E-mail: quanrc@126.com.
6. Department of pathoanatomy, College of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121000, Liaoning province, China,E-mail:simonl308@163.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cells, Cultured;
Human Umbilical Vein Endothelial Cells;
Humans;
Integrin beta3;
Signal Transduction
- From:
Journal of Experimental Hematology
2021;29(2):567-573
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the damaging of human umbilical vein endothelial cells (HUVEC) induced by antiplatelet integrin β3 antibodies in vitro.
METHODS:The serum from 36 chronic ITP patients were collected, flow cytometry and monoclonal antibody specific immobilization of platelet antigen (MAIPA) assay were used to collect antiplatelet integrin β3 antibodies from the serum of the patients. After HUVEC were treated by ITP patient serum (PS) containing anti-integrin β3 antibodies, the cell damage was detected by Lactate dehydrogenase (LDH) assay, cell apoptosis was detected by flow cytometry, the expression of apoptosis-related gene Bax was detected by Reverse transcription-Quantitative real-time PCR (RT-qPCR), and expression of Apoptosis-related signaling pathway protein Akt and related protein Bax were detected by Western blot. HUVEC were treated by PS combined with Akt activator SC79, the cells damage were detected by LDH assay, apoptosis of the cells were detected by flow cytometry, the expression of apoptosis-related gene Bax was detected by RT-qPCR.
RESULTS:Among 36 cases of serum from the chronic ITP patients, 5 patients' serum containing anti-integrin β3 antibodies were collected. After HUVEC was treated by PS, the viability of LDH was significant increased（P<0.05）, so as for the apoptosis of the cells（P<0.05）, the expression of gene and protein of Bax was increased up-regulated（P<0.05）, the protein expression of pAkt was down-regulated（P<0.05）. Comparing with HUVEC cultured with PS alone, the viability of LDH of HUVEC treated by PS combined with SC79 was significantly reduced（P<0.05）, so as for the apoptosis of the cells（P<0.05）, and gene expression of Bax was significantly decreased（P<0.05）.
CONCLUSION:Anti-integrin β3 serum can cause the damage and apoptosis of HUVEC through Akt signaling pathway，the apoptotic effects of anti-integrin β3 antibodies to HUVEC was effectively reversed by SC79.
