Optimization of Mouse Model of Aplastic Anemia Induced by Pan T Lymphocytes Combined with Irradiation.
10.19746/j.cnki.issn.1009-2137.2021.02.040
- Author:
Yu-Jie BIAN
1
;
Wei-Wang LI
1
;
Meng-Ke LI
1
;
Bi-Chen WANG
1
;
De-Yang SHI
1
;
Jun SHI
1
;
Wei-Ping YUAN
1
;
Ya-Jing CHU
2
Author Information
1. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China,E-mail:chuyajing@ihcams.ac.cn.
- Publication Type:Journal Article
- MeSH:
Anemia, Aplastic;
Animals;
Bone Marrow;
Bone Marrow Cells;
CD8-Positive T-Lymphocytes;
Humans;
Mice;
Mice, Inbred C57BL
- From:
Journal of Experimental Hematology
2021;29(2):557-566
- CountryChina
- Language:Chinese
-
Abstract:
METHODS:To establish the acquired aplastic anemia mouse model through the X-ray irradiation in combination with lymphocytes injection. AA Group: the purified Pan T lymphocytes from the spleen of C57BL/6J mice were enriched and injected to the mice through tail vein(5×10
RESULTS:Compared with 4, 5 Gy irradiated mice in AA groups, the survival time of 3 Gy irradiated AA groups was significantly prolonged. 3, 4 and 5 Gy X-ray irradiation combined with Pan T lymphocyte injection could successfully induced severe reduction of red blood cells, blood neutrophils, and platelets, severe reduction of bone marrow nucleated cells, severe bone marrow hematopoietic failure, and the significant expansion of T lymphocytes ratio in the bone marrow. CD4
CONCLUSION:3, 4 and 5 Gy X-ray irradiation combined with 5×10
