Analysis of Serum Metabonomics in Patients with Multiple Myeloma Based on Liquid Chromatography-Mass Spectrometry.
10.19746/j.cnki.issn.1009-2137.2021.02.033
- Author:
Xiao-Meng XU
1
;
Xiang-Tu KONG
2
;
Hui YU
2
;
Xiao-Li CHEN
2
;
Peng-Jun JIANG
3
;
Hai-Wen NI
4
Author Information
1. Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China.
2. Department of Hematology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, Jiangsu Province, China.
3. Department of Hematology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, Jiangsu Province, China,E-mail: jpj2000@sina.com.
4. Department of Hematology, Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, Jiangsu Province, China,E-mail: 1499169641@qq.com.
- Publication Type:Journal Article
- MeSH:
Biomarkers;
Chromatography, Liquid;
Discriminant Analysis;
Humans;
Mass Spectrometry;
Metabolomics;
Multiple Myeloma
- From:
Journal of Experimental Hematology
2021;29(2):520-524
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the changes of serum metabolites in patients with multiple myeloma (MM) by metabonomics, and explore the potential biomarkers for diagnosis, prognosis, and progression of MM.
METHODS:Serum samples were collected from 26 patients with MM and 50 healthy controls. The data detected by liquid chromatography-mass spectrometry (LC-MS) was input into SIMCA-14.0 software for multivariate statistical analysis. Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze the changes of metabolites.
RESULTS:The metabolic change of uric acid and trans-vaccenic acid in serum samples of MM patients was 9.39 times and 2.77 times of these in healthy people, respectively, which were significantly higher than those of healthy people, and the difference was statistically significant(P<0.01).
CONCLUSION:Uric acid and trans-vaccenic acid are expected to be important metabolic indicators for the diagnosis, prognosis, and efficacy evaluation of MM, thus providing some clues for the pathogenesis of MM.
