PX-12 Promoting Apoptosis of Multiple Myeloma Cell Line H929 Induced by Bortezomib.
10.19746/j.cnki.issn.1009-2137.2021.02.032
- Author:
Quan-De LIN
1
;
Yan YAN
1
;
Qing LIU
1
;
Jian-Wei DU
1
;
Xue GAO
1
;
Wen-Li ZUO
1
;
Bai-Jun FANG
1
;
Yu-Fu LI
1
;
Xu-Dong WEI
1
;
Yong-Ping SONG
2
Author Information
1. Henan Cancer Hospital, Affiliated Cancer Hospital of Zhengzhou University, Henan Institute of Hematology, Zhengzhou 450008, Henan Province, China.
2. Henan Cancer Hospital, Affiliated Cancer Hospital of Zhengzhou University, Henan Institute of Hematology, Zhengzhou 450008, Henan Province, China,E-mail: songyp357@126.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Bortezomib/pharmacology*;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Multiple Myeloma
- From:
Journal of Experimental Hematology
2021;29(2):515-519
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect of PX-12 on apoptosis of multiple myeloma (MM) cell line induced by bortezomib.
METHODS:MM cell line H929 cells were divided into PX-12 group, bortezomib group, combination group, and control group. 5.0 μmol/L PX-12, 20 nmol/L bortezomib, combination of the two drugs, and DMSO were given to the above mentioned group, respectively. After culture for 24, 48, and 72 hours, the changes of cell viability were observed, the MM cell activity was detected by MTT method, and the cell cycle distribution and apoptosis of each group was detected by flow cytometry. The intracellular ROS level was measured by H
RESULTS:MTT assay showed that after culture for 72 hours, the activity of H929 cells in PX-12 group (P<0.05) and bortezomib group (P<0.01) was significantly lower than that in the control group, while that in the combination group was decreased most significantly (P<0.01). After culture for 48 hours, cells in G1 phase in PX-12 group was decreased to 40%, while cells in S phase and G
CONCLUSION:PX-12 can increase the apoptosis of MM cell line H929 induced by bortezomib, which may be caused by increasing of ROS level.
