Effects of Long Non-coding RNA-TUC338 on the Migration and Proliferation of Lymphoma Cells via PI3K/AKT Signaling Pathway.
10.19746/j.cnki.issn.1009-2137.2021.02.029
- Author:
Zhen-Wei JIA
1
;
Yan LI
2
;
Xiao-Yang KONG
1
;
Hong-Bo ZHAO
1
;
Zhi-Feng YANG
1
;
Jing-Wei YE
1
;
Gui-Rong CUI
1
;
Jian-Min LUO
1
Author Information
1. Department of Hematology, Handan First Hospital, Handan 056002, Hebei Province, China.
2. Department of Hematology, Handan First Hospital, Handan 056002, Hebei Province, China,E-mail: handanliyan68@sohu.com.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Humans;
Phosphatidylinositol 3-Kinases/metabolism*;
Proto-Oncogene Proteins c-akt/metabolism*;
RNA, Long Noncoding/genetics*;
Signal Transduction
- From:
Journal of Experimental Hematology
2021;29(2):494-499
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of long non-coding RNA-TUC338 on the proliferation and migration of lymphoma cells.
METHODS:The expression of TUC338 in different lymphoma cells was detected by fluorescence quantitative PCR, cell proliferation by sulforhodamine B (SRB) assay, migration of lymphoma cells by transwell assay, and protein expression in PI3K/AKT signaling pathway by Western blot.
RESULTS:The expression levels of TUC338 in lymphoma cells Daudi, U937, BC-3, and Raji significantly increased in comparison with human normal T lymphocytes H9 (t=13.277, 10.103, 16.200, and 26.687, P=0.002, 0.005, 0.001, and 0.000). Compared with NC-siRNA group, the number of cells crossing the chamber of TUC338-siRNA group was significantly reduced (t=30.508, P=0.000), the protein expression levels of p-PI3K and p-AKT significantly decreased (t=16.872 and 18.371, P=0.000 and 0.000), and OD
CONCLUSION:The expression of TUC338 significantly increases in lymphoma cells, and silence of TUC338 effectively inhibits the activation of PI3K/AKT signaling pathway, thereby inhibiting the proliferation and migration of lymphoma cells, which has a potential application value in diagnosis and treatment of lymphoma.
