Up-Regulation of Bax/BCL-2 Ratio by 2-Methoxyestradiol Induces Apoptosis in Lymphoma Raji Cells.
10.19746/j.cnki.issn.1009-2137.2021.02.028
- Author:
Xue-Ya ZHANG
1
;
Xi-Zhe GUO
1
;
Shi-Xin WU
2
Author Information
1. Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
2. Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China,E-mail:59212080@sina.com.
- Publication Type:Journal Article
- MeSH:
2-Methoxyestradiol;
Apoptosis;
Caspase 3/metabolism*;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Lymphoma;
Proto-Oncogene Proteins c-bcl-2/metabolism*;
Up-Regulation;
bcl-2-Associated X Protein
- From:
Journal of Experimental Hematology
2021;29(2):489-493
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of 2-methoxyestradiol (2-ME2) to lymphoma Raji cells and its mechanism.
METHODS:Different concentrations of 2-ME2 were used to treat lymphoma Raji cells. CCK8 method was used to detect the effect of 2-ME2 to proliferation of Raji cells. Flow cytometry FITC/PI double labeling method was used to detect early apoptosis of the cells. Western blotting was used to detect the effect of 2-ME2 to the expression of BCL-2, Bax, Caspase-3 and C-myc proteins in Raji cells.
RESULTS:2-ME2 significantly inhibited the proliferation of Raji cells. The inhibition rate increased with the increasing of drug concentration, and increased significantly with the prolongation of drug treatment time (r=0.9215). Flow cytometry FITC/PI double staining showed that the apoptotic rate of 2.5 μmol/L 2-ME2 treatment group was (33.79±1.63) %, while the apoptosis rate of the 48 h group was (51.90±2.72) %, and that of the control group was (7.08±0.36) %. After treated with 2.5 μmol/L 2-ME2 for 12 h, the expression of Bax protein was up-regulated, BCL-2 protein was down-regulated, caspase-3 protein expression was up-regulated, and C-myc protein expression was down-regulated, all of them showed a time-dependent relationship.
CONCLUSION:2-ME2 shows obvious inhibitory effect on lymphoma Raji cells in a dose- and time-dependent manner. Its mechanism of treatment on lymphoma Raji cells may be related to up-regulation of Bax/BCL-2 ratio and activation of Caspase-3 to induce apoptosis in cancer cells. Down-regulation of C-myc protein expression also participates in the apoptotic process.
