Effect of Etoposide on Elimination of Chronic Myeloid Leukemia Stem Cells by Imatinib in Vivo.
10.19746/j.cnki.issn.1009-2137.2021.02.014
- Author:
Xiang-Jie CHEN
1
;
Qing-Qing WU
1
;
Man-Yu LIU
2
;
Wei-Zhang WANG
3
Author Information
1. School of Life Sciences and Biopharmaceutical,Guangzhou 510006, Guangdong Province, China,Guangdong Province Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou 510006, Guangdong Province, China.
2. Guangdong Province Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou 510006, Guangdong Province, China,School of Food Sciences, Guangdong Pharmaceutical University,Guangzhou 510006, Guangdong Province, China.
3. School of Life Sciences and Biopharmaceutical,Guangzhou 510006, Guangdong Province, China,Guangdong Province Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou 510006, Guangdong Province, China,E-mail: wwzss@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Drug Resistance, Neoplasm;
Etoposide;
Fusion Proteins, bcr-abl;
Imatinib Mesylate;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy*;
Mice;
Stem Cells
- From:
Journal of Experimental Hematology
2021;29(2):395-402
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of etoposide (ETO) on elimination of chronic myeloid leukemia (CML) stem cells by imatinib mesylate(IM) in vivo.
METHODS:SCL-tTA/BCR-ABL mice were used as CML animal model. Flow cytometry was used to assess the effect of ETO alone or in combination with IM on the number of leukemia stem cell （LSC） in bone marrow and spleen, and peripheral blood neutrophils in CML mice and normal control FVB mice.
RESULTS:The results showed that in CML mice, the number and proportion of LSC in bone marrow and the proportion of neutrophils in peripheral blood decreased significantly after ETO and IM combined treatment, and the degree of decrease was more significant than that of both alone. While in wild type FVB mice, the combination of ETO and IM showed no significant effect on the number and proportion of LSK cells in bone marrow and the proportion of neutrophils in spleen.
CONCLUSION:ETO can selectively enhance elimination of CML LSC by IM in vivo.
