Primary Study on Chronic Myeloid Leukemia in NCG Mice from Qinba Mushroom.
10.19746/j.cnki.issn.1009-2137.2021.02.012
- Author:
Ming-Xia CHENG
1
,
2
;
Jing QI
2
,
3
;
Lian-Ping ZHAO
2
,
4
;
Li LYU
5
;
Xue CHEN
2
,
6
;
Gao LI
1
,
2
;
Kun-Tian BAI
1
,
2
;
Cai-Juan HAN
1
,
2
;
Yan-Qing SUN
1
,
7
,
8
Author Information
1. Department of Hematology, Gansu Provincial Hospital
2. Lanzhou 730000, Gansu Province, China.
3. Stomatology Center of Gansu Provincial Hospital
4. Department of Clinical Teaching，Gansu Provincial Hospital
5. Department of Clinical Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, Gansu Province, China.
6. Department of Clinical Teaching, Gansu Provincial Hospital
7. Lanzhou 730000, Gansu Province, China,Department of Clinical Teachingy, Gansu Provincial Hospital
8. Lanzhou 730000, Gansu Province, China,E-mail:18394496255@163.com.
- Publication Type:Journal Article
- MeSH:
Agaricales;
Animals;
Apoptosis;
Cell Proliferation;
Humans;
K562 Cells;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
Mice
- From:
Journal of Experimental Hematology
2021;29(2):381-388
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The present study was to evaluate the anti-tumor effects of acidic RNA protein complex (FA-2-b-β) extracted from the wild edible Qinba mushroom in inducing of apoptosis and immunoregulation of tumor cell.
METHODS:Cell proliferation inducing rate of FA-2-b-β to K562 cell was measured using CCK-8. Apoptosis rate was detected by using flow cytometry. Chronic myeloid leukemia model was developed by tail vein injection/subcutaneous inoculation of K562 cells in NCG mice. The tumor burden of mice was observed. The general condition of the mice was monitored twice daily. The peripherivcal full blood counts of mice was tested daily. RT-qPCR and Western blot was FA-2-b-β performed to determine involvement of apoptotic-related gene and protenin, Immunofluorescence and immunohistochemistry was used to detected the expression of CD3, CD4 and CD8.
RESULTS:The proliferation and apoptosis of K562 cell could be inhibitied and induced by FA-2-b-β, there was 100% successful in the tumor formation in vivo, after treated by drug for 21 days there were significantly increased peripheral leucocytes, but decreased hemoglobin of mice treated by FA-2-b-β as compared with those in control group. The CD3, CD4 and CD8 showed positive in mice, and the propotation was imbalance, but it showed reserved after treated by FA-2-b-β.
CONCLUSION:FA-2-b-β is strong anti-leukemia effect in vitro and in vivo, suggesting the traditional Chinese medicine maybe contribute to the anti-cancer and immunoregulation research.
