Platelet HPA Typing of Platelet Donors in Zhangjiakou Area.
10.19746/j.cnki.issn.1009-2137.2021.01.038
- Author:
Wen-Jing JIANG
1
;
Yun-Peng XU
2
;
Fei LI
3
;
Ping LI
4
;
Shao-Ping KANG
1
;
Li-Hong LI
1
;
Yi-Hao WANG
1
Author Information
1. College of Laboratory Medicine in Hebei North University,Zhangjiakou 075000, Hebei Province, China.
2. Blood Center of Zhangjiakou Area in Hebei Province,Zhangjiakou 075000, Hebei Province, China.
3. Department of Blood Transfusion, The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000, Hebei Province, China.
4. College of Laboratory Medicine in Hebei North University,Zhangjiakou 075000, Hebei Province, China,E-mail: 504670825@qq.com.
- Publication Type:Journal Article
- MeSH:
Antigens, Human Platelet/genetics*;
Blood Donors;
Gene Frequency;
Genotype;
Humans;
Polymorphism, Genetic
- From:
Journal of Experimental Hematology
2021;29(1):233-238
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the polymorphism of the HPA1-5,15 system of the donors in Zhangjiakou area.
METHODS:DNA was extracted from the blood samples of the donors, PCR- SSP method was used to divide HPA1-6, 15 genotype. The gene frequency and genotype frequency were calculated, compared with the difference and regiahal specificity of the populations in our country and foregiens was compared other populations.
RESULTS:The gene expression in the HPA-1, HPA-2 and HPA-4 systems were all homozygous aa, and the donors who expressed homozygous bb was not exessed. Among them, one heterozygous ab expression was found in both HPA-1 and HPA-4 systems (1%), and 14 cases of heterozygous ab expression were found in HPA-2 system (14%). The gene expression in the HPA-5 system was mainly homozygous aa (98%), and a very few expressed homozygous bb (2%) was found. The degree of heterozygosity of gene expression in the HPA-3 and HPA-15 systems was relatively high. The proprotion of the expression of aa, ab and bb in the HPA-3 system was respectively 46%, 40% and 14%, the proprotion of the expression of aa, ab and bb in the HPA-15 system was respectively 21%, 64% and 15%.
CONCLUSION:The gene frequency of platelet-specific antigen HPA1-5,15 system in zhangjiakou region shows local characteristics. The heterozygosity degree of gene expression in the HPA-3 and HPA-15 systems are both high, suggesting that they are more likely to result in alloimmunization and ineffective platelet transfusion, which should be pays attention to.
