Costunolide Induces Apoptosis of K562/ADR Cells through PI3K/AKT Pathway.
10.19746/j.cnki.issn.1009-2137.2021.01.011
- Author:
Hong CAI
1
;
Hong HU
2
Author Information
1. Department of Clinical Laboratory Examination, The Second Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China.
2. Department of Clinical Laboratory Examination, The Second Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China,E-mail: 120194403@qq.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Proliferation;
Humans;
K562 Cells;
Phosphatidylinositol 3-Kinases;
Proto-Oncogene Proteins c-akt;
Sesquiterpenes
- From:
Journal of Experimental Hematology
2021;29(1):68-71
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of costunolide on the proliferation and apoptosis of human chronic myeloid leukemia drug resisitant cell line K562/ADR and its mechanism.
METHODS:The proliferation of the cells was assessed by CCK-8 assay, while flow cytometry was used to detect the apoptosis of the cells. The related-proteins were detected by using Western blot.
RESULTS:The proliferation of K526/ADR cells was significantly inhibited by costunolide in a dose-dependent manner (r=0.9886) after treated by 0.01, 0.1, 0.25, 0.5, 1, 2.5, 5, 10, 25, 50 and 100 μmol/L costunolide for 72 h, and IC
CONCLUSION:Costunolide could inhibit the proliferation and apoptosis of K562/ADR cells through regulation of PI3K/AKT pathway.
