Analysis on Polymorphism of Platelet Antigen Gene in Shandong Han Population.
10.19746/j.cnki.issn.1009-2137.2020.06.044
- Author:
Yan LIU
1
;
Hai-Feng ZHU
1
;
Wen-Ben QIAO
1
;
Yi ZHANG
1
;
Yong-Hong SONG
1
;
Xiang-Min NIE
1
;
Chuan-Fu ZHU
1
;
Pei-Cong ZHAI
2
Author Information
1. HLA Laboratory, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
2. HLA Laboratory, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China,E-mail: sd1866011@163.com.
- Publication Type:Journal Article
- MeSH:
Alleles;
Antigens, Human Platelet/genetics*;
Gene Frequency;
Genotype;
Humans;
Polymorphism, Genetic
- From:
Journal of Experimental Hematology
2020;28(6):2066-2070
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the Polymorphism of the human platelet antigen(HPA) gene 1-17 and human leukocyte antigen(HLA) gene-A and B locus in Shandong Han population.
METHODS:A total of 962 samples from routine voluntary platelet donors were genotyped for HPA1-17 system and HLA-A site, B by PCR-SSP and PCR-SSOP respectively．Gene frequencies were calculated by counting. HPA1-17 and HLA genotype combinations were analyzed by Arelequin 3.5.
RESULTS:The gene frequencies of HPA-la, -1b, HPA-2a, -2b, HPA-3a, -3b, HPA-4a, -4b, HPA-5a, -5b, HPA-6a, -6b, HPA-15a, -15b were 0.9918, 0.0082, 0.9419, 0.0592, 0.5841, 0.4174, 0.9969, 0.0031, 0.9892, 0.0108, 0.9835, 0.0175,0.5488 and 0.4512, respectively． The most common HPA genotype combination was HPA-(1, 2, 4, 5, 6, 7-14, 16, 17) aa-3ab-15ab (0.2048). Moreover, HLA-A*2(0.3094) and HLA-B*13(0.1513) showed the highest frequency in their respective locus. The most common HLA genotype combination was HLA-A*2-B*13(0.1397) .
CONCLUSION:Distributions of HPA and HLA show high polymorphism in Shandong Han population. The ethnic and territorial difference of HPA distribution is also confirmed. It is imperative to establish local genetic database of volunteer platelet donors.
