Effects of IL-27 on Th17 Cells in Patients with Henoch-Schönlein Purpura.
10.19746/j.cnki.issn.1009-2137.2020.06.042
- Author:
Ya-Feng LI
1
;
Hai-Long XIA
2
Author Information
1. Department of Hematology, The First Affiliated Hospital of Anhui Medical University，Hefei 230022, Anhui Province, China.
2. Department of Hematology, The First Affiliated Hospital of Anhui Medical University，Hefei 230022, Anhui Province, China,E-mail: xhl1999cn@163.com.
- Publication Type:Journal Article
- MeSH:
Humans;
Interleukin-27;
Leukocytes, Mononuclear;
Patients;
Purpura, Schoenlein-Henoch;
Th17 Cells
- From:
Journal of Experimental Hematology
2020;28(6):2051-2055
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of IL-27 on Th17 cells in patients with henoch-schönlein purpura（HSP） in order to further elucidate the pathogenesis.
METHODS:Fifty patients with HSP treated in our hospital from April 2019 to July 2019 were selected as HSP group, and 30 volunteers underwent physical examination at the same time were selected as control group. The proportion of Th17 cells in peripheral blood of HSP group and healthy control group was determined by flow cytometry (FCM). A total of 27 HSP patients were selected, and candidate peripheral blood mononuclear lymphocytes (PBMC) were co-cultured with exogenous rhIL-27, and the ratio of Th17 cells was detected by flow cytometry.
RESULTS:The proportion of Th17 cells in the peripheral blood of HSP patients with acute phase was (1.57±0.54)%, which was significantly higher than that of the control group (0.86±0.40)% (t=-6.298, P<0.001), and the proportion of Th17 cells was decreased significantly after rhIL-27 co-culture (1.39%±0.52% vs 0.98%±0.44%)(P<0.05).
CONCLUSION:IL-27 can reduce the level of Th17 cells in patients with HSP, which may be involved in the pathogenic process of HSP and play a protective role in the development of the disease.
