Antiproliforative effect and HLA-DR induction of recombinant gamma interferon on cultured human keratinocytes.
- Author:
Chee Woo YANG
;
Woo Young SIM
;
Mu Hyoung LEE
;
Jai Kyung PARK
;
Choong Rim HAW
- Publication Type:Original Article
- Keywords:
Recombinant;
IFN--y;
HLA-DR
- MeSH:
HLA-DR Antigens*;
Humans*;
Interferon-gamma;
Interferons*;
Keratinocytes*
- From:Korean Journal of Dermatology
1991;29(1):1-7
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The authors investigated the antiproliferative effect and expression of HLA-DR an- tigen by recombinant gamma-interferon (r-IFN-y) on cultured human keratinocytes (KC). The results were as follows, 1. From 10l.J/ml of r-1FN-p exposure, the proliferation of KC decreased in a concentration dependent fashion. But there was little difference of antiproliferative effect above 30U/ml of r-IFN-y exposure. 2. The expression of HLA-DR antigen on KC increased in a concentration and time dependent fashion of r-IFN-p exposure. E3ut t,here was little difference of HLA-DR antigen expression on KC above 30tJ/ml and most of HLA-DR antigen were expressed within 48hr. 3. The opt,imal condition for HLA-DR antigen induction on KC by r-IFN-p was likely t,hat HLA-DR KC was observed at 48hr under the our exposure of 30U/ml of r-IFN p. 4. After 4hr exposure of 30U/ml of r-IFN-p, KC expresed HLA-BR. antigen, reaching a maximum intensity at 3 days. At, 7 days, the loss of HI A-DR KC showed over 90% of maximum intensity.
