The Molecular Mechanism of LncRNA-CASC2/miR-155-5p/APC Axis Regulating the Progression of Non-Hodgkin Lymphoma.
10.19746/j.cnki.issn.1009-2137.2020.06.024
- Author:
Chun-Jiao ZHENG
1
;
Zhi-Ming WU
1
;
Yan-Shuang WANG
1
;
Yun-Xiu HOU
2
Author Information
1. Department of Laboratory Medicine, The Second Affiliated Hospital of Hainan Medical College, Haikuo 570311, Hainan Province, China.
2. Department of Laboratory Medicine, The Second Affiliated Hospital of Hainan Medical College, Haikuo 570311, Hainan Province, China,E-mail:nvgout@163.com.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Gene Expression Regulation, Neoplastic;
Humans;
Lymphoma, Non-Hodgkin/genetics*;
MicroRNAs/genetics*;
RNA, Long Noncoding/genetics*;
Tumor Suppressor Proteins/genetics*
- From:
Journal of Experimental Hematology
2020;28(6):1939-1945
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of lncRNA-CASC2 (CASC2) /miR-155-5p/APC axis to the progression of non-Hodgikn lymphoma (NHL).
METHODS:The expression level of CASC2 and miR-155-5p in NHL cell lines were examined by qRT-PCR. Dual-luciferase reporter gene assay was used to verify the relationship between miR-155-5p, CASC2 and APC. The effects of CASC/miR-155-5p/APC axis to the proliferation, invasion and apoptosis of NK-92 cells were detected by MTT, Transwell assay and flow cytometry assay, respectively.
RESULTS:CASC2 was downregulated in NHL cell lines. Overexpression of CASC2 could inhibit the proliferation and invasion of NK-92 cells, and promote its apoptosis. Dual-luciferase reporter gene assay confirmed that there was a targeting relationship between miR-155-5p, CASC2 and APC. The restoration experiments proved that knockdown of both miR-155-5p and CASC2 or APC could restore the inhibitory effect of miR-155-5p silencing to the biological behavior of NK-92 cells.
CONCLUSION:Overexpression of CASC2 suppresses the proliferation and invasion of NK-92 cells, promote the apoptosis of NK-92 cells via targeting miR-155-5p and upregulating APC expression.
