Effects of Soluble CD40 Ligand on Non-Hodgkin Lymphoma Cells and Its Relative Mechanism.
10.19746/j.cnki.issn.1009-2137.2020.06.023
- Author:
Zhong-Xin FENG
1
;
Ji-Shi WANG
2
;
Qi CHEN
3
Author Information
1. Department of Hematology of Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China,Guizhou Medical University Graduate School, Guiyang 550004, Guizhou Province, China.
2. Department of Hematology of Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China.
3. Department of Hematology of Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China,E-mail: zyfychenqi@163.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
CD40 Ligand;
Cell Line, Tumor;
Cell Proliferation;
Family;
Humans;
Lymphoma, Non-Hodgkin
- From:
Journal of Experimental Hematology
2020;28(6):1933-1938
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of soluble CD40 ligand (sCD40L) on the proliferation, apoptosis, and cell cycle of human non-Hodgkin lymphoma (NHL) cells, and analyze its possible mechanism.
METHODS:NHL CA46 cell and Raji cell were treated with different concentrations of sCD40L for 48 h, CCK-8 was used to detect the effect of sCD40L on cell proliferation in vitro, flow cytometry on apoptosis and cycle of NHL cells, and Western blot on the expression of PTEN, BCL-2, and BAX in NHL cells.
RESULTS:Compared with the control group, 4 and 8 μg/ml sCD40L could significantly inhibit the proliferation of lymphoma Raji cell and CA46 cell (P<0.05). The test results of flow cytometry showed that 4 μg/ml sCD40L could significantly promote the apoptosis of CA46 and Raji cells, and significantly inhibit the S phase proportions (P<0.05). Western blot results showed that sCD40L could promote the expression of PTEN and BAX, while inhibit the expression of BCL-2 (P<0.05).
CONCLUSION:sCD40L can promote the apoptosis and inhibit the proliferation of NHL cells through the PTEN signaling pathway.
