Inhibitory Effects of Agaricus Blazei Murrill (FA-2-b-β) on Proliferation of Chronic Myeloid Leukemia Cells in vivo and Its Mechanism.
10.19746/j.cnki.issn.1009-2137.2020.06.016
- Author:
Ming-Xia CHENG
1
,
2
,
3
;
Shang-Rui YU
4
,
5
;
Zhan-Dong WANG
2
,
3
;
Kun-Tian BAI
2
,
3
;
Dong-Ping WANG
6
;
Juan LI
6
;
Shi-Dong LIU
7
;
Yan-Qing SUN
8
,
9
,
10
;
Li DONG
11
Author Information
1. Department of Hematology, Gansu Provincial People's Hospital
2. Department of Clinical Medicine, Gansu University of Chinese Medicine
3. Lanzhou 730000, Gansu Province, China.
4. Department of Digestion, The Second Hospital of Lanzhou University
5. Lanzhou 730000, Gansu Province, China.
6. Department of Hematology, Gansu Provincial People's Hospital Lanzhou 730000, Gansu Province, China.
7. Department of Cardiovascular Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
8. Department of Hematology, Gansu Provincial People's Hospital
9. Department of Clinical Medicine, Gansu University of Chinese Medicine
10. Lanzhou 730000, Gansu Province, China E-mail:18394496255@163.com.
11. Department of Hematology, Gansu Provincial People's Hospital Lanzhou 730000, Gansu Province, China E-mail: donglijustgood@126.com.
- Publication Type:Journal Article
- MeSH:
Agaricus;
Animals;
Apoptosis;
Cell Proliferation;
Humans;
Imatinib Mesylate;
K562 Cells;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
Mice
- From:
Journal of Experimental Hematology
2020;28(6):1885-1891
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigated the anti-tumor in vivo effect and mechanism of the acid RNA protein complex (FA-2-b-β) of Agaricus blazei Murrill extract.
METHODS:CCK-8 method was used to detected the inhibitory effect of FA-2-b-β on proliferation of primary CML cells from newly diagnosed CML patients, the CML mouse model was established by trail-venous injection of primary CML cells, and the survival time, blood cell count and body weight were observed, the immunoflouresence and immunehistochemistry analysis, RT-qPCR, Western bolt were used to detemine the expression of caspase-3 signal pathway-related apoptosis genes and proteins.
RESULTS:The experiments in vitro showed that the proliferative inhibitory rate in drug-treated group increased with concentration- and time-dependent manner (r
CONCLUSION:The FA-2-b-β can induce apoptosis of primary CML cells and prolong the survival time of CML model mouse, which may be related with the caspase-3 signal pathway related genes and proteins.
