Hsa-let-7b-5p Inhibits Proliferation of Human Leukemia THP-1 Cells via FTO/m
10.19746/j.cnki.issn.1009-2137.2020.06.014
- Author:
Dan-Sen WU
1
;
Cai-Fa ZHENG
2
;
Yong-Ping ZENG
2
;
Jing LIN
2
;
Jia-Long CHEN
2
;
Song-Jing SHI
3
Author Information
1. Fujian Provincial Hospital，Shengli Clinical College of Fujian Medical University， Fuzhou 350001, Fujian Province, China,E-mail：wuds2006@126.com.
2. Fujian Provincial Hospital，Shengli Clinical College of Fujian Medical University， Fuzhou 350001, Fujian Province, China.
3. Fujian Provincial Hospital，Shengli Clinical College of Fujian Medical University， Fuzhou 350001, Fujian Province, China,E-mail：serena10@sina.com.
- Publication Type:Journal Article
- MeSH:
Alpha-Ketoglutarate-Dependent Dioxygenase FTO/genetics*;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Leukemia;
Leukocytes, Mononuclear;
MicroRNAs/genetics*;
Signal Transduction;
THP-1 Cells
- From:
Journal of Experimental Hematology
2020;28(6):1873-1879
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the down-regulation effect of let-7b-5p on the expression of FTO in acute myeloid leukemia cell line THP-1 and inhibitory effect on THP-1 proliferation via m
METHODS:The acute myeloid leukemia cell line THP-1 and the normal human peripheral blood mononuclear cells (PBMNC) were selected as subjects. The expression of let-7b-5p and FTO mRNA in those cells was detected by qPCR, further the expression of FTO protein in those cells was detected by Western blot. And, the luciferase reporter gene assay was used to verify the targeting effect of let-7b-5p on FTO. Finally, THP-1 cells were transfected respectively with let-7b-5p mimic, and PBMNC with let-7b-5p inhibitor, there after the C-MYC mRNA m
RESULTS:Compared with PBMNC, the expression of let-7b-5p in THP-1 significantly decreased, while the expression of FTO was significantly increased (P<0.05). After transfection with let-7b-5p mimic combined with FTO 3'-UTR, the luciferase activity of transfected THP-1 cells significantly decreased, but the luciferase activity significantly increased after transfection with mutant 3'-UTR, which was significantly different from the negative control group(blank vector) (P<0.05). Let-7b-5p inhibitor down-regulated c-MYC mRNA m
CONCLUSION:Human acute myeloid leukemia cell line THP-1 low expresses the let-7b-5p, which regulates c-MYC expression through let-7b-5p-/FTO-/m
