Effects and Mechanism of PARP Inhibitor Olaparib on the Expression of NKG2D Ligands in HL-60 Cells.
10.19746/j.cnki.issn.1009-2137.2020.06.006
- Author:
Zhi-Chao ZHU
1
;
Yu BAI
1
;
Xu-Zhang LU
2
;
Chun-Jian QI
3
Author Information
1. Laboratory Center, Changzhou No.2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, Changzhou 213000, Jiangsu Province, China.
2. Department of Hematology, Changzhou No.2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, Changzhou 213000, Jiangsu Province, China.
3. Laboratory Center, Changzhou No.2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, Changzhou 213000, Jiangsu Province, China E-mail: qichunjian@njmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Cytotoxicity, Immunologic;
HL-60 Cells;
Histocompatibility Antigens Class I;
Humans;
Ligands;
NK Cell Lectin-Like Receptor Subfamily K;
Phthalazines;
Piperazines;
Poly(ADP-ribose) Polymerase Inhibitors
- From:
Journal of Experimental Hematology
2020;28(6):1826-1830
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the regulatory effects of Olaparib on natural killer cell activating receptor (NKG2D) ligands expression on human acute myeloid leukemia (AML) cell line HL-60, and to explore the molecular mechanism of Olaparib on HL-60 cells.
METHODS:After HL-60 cells in logarithmic growth phase were treated with Olaparib at different concentrations for different times (24, 48 h), the expression of NKG2D ligand on the surface of HL-60 cells was detected by flow cytometry. Western blot was used to dectect the expression of ERK expression in HL-60 cells. The killing effect of NK cells to HL-60 cells was detected by CFSE/PI method.
RESULTS:10 μmol/L Olaparib could upregulate the expression of NKG2D ligand on the surface of HL-60 cell at 24 and 48 hours, while 5 μmol/L Olaparib could induce up-regulation of the expression of ULBP-2 and ULBP-3 at 48 hours. Western blot analysis showed that ERK phosphorylation of HL-60 cells was enhanced after treating with Olaparib. The killing effect of NK cells to HL-60 cells could be enhanced by Olaparib, however, ERK inhibitor could suppress the killing effect of NK cells to HL-60 cells.
CONCLUSION:Olaparib can upregulate NKG2D ligands expression on the surface of HL-60 cells and enhance the cytotoxicity of NK cell to HL-60 cells. The mechanism may be related to Olaparib promoting ERK phosphorylation expression.
