Protective effect of transient receptor potential melastatin 2 inhibitor A10 on oxygen glucose deprivation/reperfusion model.
10.3724/zdxbyxb-2021-0044
- Author:
Jianguo NIU
1
;
Xingyu LIU
1
;
Minghua HUANG
1
;
Xiafei YU
1
;
Fangfang LI
1
;
Wei YANG
1
;
Kang MA
1
;
Zhuoqun HUANG
1
Author Information
1. Department of Human Anatomy，Histology and Embryology，School of Basic Medicine，Ningxia Medical University，Yinchuan 750004，China.
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cleaved caspase 3;
Mitochondrial membrane potential;
Oxygen glucose deprivation/reperfusion;
Reactive oxygen species;
SH-SY5Y cells;
Transient receptor potential melastatin 2 channel
- MeSH:
Apoptosis;
Benzeneacetamides;
Cell Survival;
Glucose;
Humans;
Oxygen/metabolism*;
Piperidones;
Reactive Oxygen Species/metabolism*;
Reperfusion;
TRPM Cation Channels
- From:
Journal of Zhejiang University. Medical sciences
2021;50(1):106-112
- CountryChina
- Language:English
-
Abstract:
:To investigate the effect of transient receptor potential melastatin 2 （TRPM2） inhibitor A10 on oxygen glucose deprivation/reperfusion （OGD/R） injury in SH-SY5Y cells．:Human neuroblastoma SH-SY5Y cells were subject to OGD/R injury，and then were divided into blank control group，model control group and A10 group randomly. The cell survival rate was detected by cell counting kit 8 （CCK-8）; the level of cellular reactive oxygen species （ROS） was detected by reactive oxygen detection kit; the mitochondrial membrane potential was detected by tetramethylrhodamine （TMRM） method; the number of apoptotic cells was detected by TUNEL apoptosis assay kit; the protein expression level of cleaved caspase 3 was detected by Western blot．:Compared with 3，20，30，50， has lower cytotoxicity and better inhibition effect on channel activity. Compared with the model control group，ROS level was reduced，the mitochondrial membrane potential was improved，the number of apoptosis cells was reduced ，and the expression of cleaved caspase 3 was significantly reduced in the A10 group（all <0.05）. : A10 can alleviate cell damage after OGD/R by inhibiting TRPM2 channel function，reducing extracellular calcium influx，reducing cell ROS levels，stabilizing mitochondrial membrane potential levels，and reducing apoptosis.
