Preparation of an anti-cotinine monoclonal antibody and its application in immunological detection.
10.3785/j.issn.1008-9292.2020.12.12
- Author:
Yajing LEI
1
;
Lifang ZHOU
1
;
Anxing WANG
1
;
Shuqing CHEN
2
Author Information
1. Hangzhou En's Gene, C&Y Pharmaceutical Group Co. Ltd, Hangzhou 310052, China.
2. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
- Publication Type:Journal Article
- Keywords:
Cotinine;
Immunochromatographic strip;
Indirect competitive enzyme linked immunosorbent assay;
Monoclonal antibody;
Urine
- MeSH:
Animals;
Antibodies, Monoclonal;
Cotinine/urine*;
Enzyme-Linked Immunosorbent Assay;
Gold Colloid;
Humans;
Mice;
Mice, Inbred BALB C;
Urinalysis/methods*
- From:
Journal of Zhejiang University. Medical sciences
2020;49(6):765-771
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare monoclonal antibody against cotinine (COT) and to establish immunoassay for detecting COT in human urinary samples.
METHODS:BALB/c mice were immunized with synthesized cotinine-bovine serum albumin (COT-BSA) to screen monoclonal antibody with technique of cell fusion. The monoclonal antibody was used for the indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold immunochromatographic strip assay for the detection of COT in human urine.
RESULTS:The monoclonal antibody against COT was identified by ic-ELISA with a 50%inhibitive concentration (IC
CONCLUSIONS:The ic-ELISA and colloidal gold immunochromatographic strip assay using the prepared monoclonal antibody against COT have been proved to be reliable for the rapid detection of COT in human urines, which may be used for monitoring of environmental tobacco smoke.
