Differentially expressed inflammatory proteins in acute gouty arthritis based on protein chip.
10.3785/j.issn.1008-9292.2020.12.09
- Author:
Guanghan SUN
1
;
Jian LIU
1
;
Lei WAN
1
;
Wei LIU
2
;
Yan LONG
1
;
Bingxi BAO
1
;
Ying ZHANG
1
Author Information
1. Department of Rheumatology and Immunology, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230031, China.
2. Department of Rheumatology and Immunology, the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China.
- Publication Type:Journal Article
- Keywords:
Acute gouty arthritis;
Biomarkers;
Inflammatory function;
Proteomics
- MeSH:
Arthritis, Gouty/diagnosis*;
Cytokines/genetics*;
Gene Expression Profiling;
Gene Expression Regulation;
Humans;
Inflammation;
Protein Array Analysis;
Proteomics
- From:
Journal of Zhejiang University. Medical sciences
2020;49(6):743-749
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect the differentially expressed inflammatory proteins in acute gouty arthritis (AGA) with protein chip.
METHODS:The Raybiotech cytokine antibody chip was used to screen the proteomic expression in serum samples of 10 AGA patients and 10 healthy individuals. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were applied to determine the biological function annotation of differentially expressed proteins and the enrichment of signal pathways. ELISA method was used to verify the differential protein expression in 60 AGA patients and 60 healthy subjects. The ROC curve was employed to evaluate the diagnostic value of differential proteins in AGA patients.
RESULTS:According to|log
CONCLUSIONS:Proteomics can be applied to identify the biomarkers of AGA, which may be used for risk prediction and diagnosis of AGA patients.
