Master genes and co-expression network analysis in peripheral blood mononuclear cells of patients with gram-positive and gram-negative sepsis.
10.3785/j.issn.1008-9292.2020.12.08
- Author:
Lu LI
1
;
Junjun FANG
2
;
Zhitao LI
2
;
Leixing SHEN
3
;
Guobin WANG
2
;
Shuiqiao FU
2
Author Information
1. Department of Clinical Pharmacy, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
2. Surgical Intensive Care Unit, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
3. School of Pharmaceutical Sciences, Zhejiang University City College, Hangzhou 310015, China.
- Publication Type:Journal Article
- Keywords:
Gram-negative bacterium;
Gram-positive bacterium;
Peripheral blood mononuclear cell;
Sepsis;
Systemic inflammatory response syndrome
- MeSH:
Biomarkers/analysis*;
Gene Expression Profiling;
Gram-Negative Bacterial Infections/physiopathology*;
Gram-Positive Bacterial Infections/physiopathology*;
Humans;
Leukocytes, Mononuclear/pathology*;
Sepsis/physiopathology*
- From:
Journal of Zhejiang University. Medical sciences
2020;49(6):732-742
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the functional pathways enriched and differentially expressed genes (DEGs) in peripheral blood mononuclear cells (PBMCs) of patients with gram-positive and gram-negative sepsis.
METHODS:Dataset GSE9960 obtained from NCBI GEO database containing PBMC samples from 16 non-infectious systematic inflammatory response syndrome (SIRS) patients, 17 gram-positive septic patients and 18 gram-negative septic patients were included in the study. Functional pathway annotations were conducted by gene set enrichment analysis and weighted gene co-expression network analysis. DEGs were filtered and master DEGs were then validated in PBMCs of gram-positive septic, gram-negative septic and non-infectious SIRS patients.
RESULTS:The enriched gene sets in gram-positive sepsis and gram-negative sepsis were significantly different. The results indicated the opposite co-expression networks in SIRS and gram-negative sepsis, and the entirely different co-expression networks in gram-positive and gram-negative sepsis. Furthermore, we validated that
CONCLUSIONS:The results indicate that there are differences in the mechanism and pathogenesis of gram-positive and gram-negative sepsis, which may provide potential markers for sepsis diagnosis and empirical antimicrobial therapy.
