Effects of delivery and storage conditions on concentrations of amino acids and carnitines in neonatal dried blood spots.
10.3785/j.issn.1008-9292.2020.10.03
- Author:
Lingwei HU
1
;
Zhenzhen HU
1
;
Jianbin YANG
1
;
Yu ZHANG
2
;
Yezhen SHI
2
;
Shasha ZHU
3
;
Rulai YANG
1
;
Xinwen HUANG
1
Author Information
1. Department of Genetics and Metabolism, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Regional Medical Center for Children, Hangzhou 310052, China.
2. Zhejiang Biosan Biochemical Technologies Co., Ltd., Hangzhou 310012, China.
3. Department of Pediatrics, Taizhou Maternal and Child Health Hospital, Taizhou 318000, Zhejiang Province, China.
- Publication Type:Journal Article
- Keywords:
Amino acids;
Carnitine;
Cold-chain transportation;
Dried blood spot testing;
Genetic and metabolic diseases screening;
Humidity;
Storage;
Temperature
- MeSH:
Amino Acids/analysis*;
Carnitine/analysis*;
Dried Blood Spot Testing/standards*;
Humans;
Humidity;
Infant, Newborn;
Neonatal Screening;
Reproducibility of Results;
Specimen Handling/standards*;
Tandem Mass Spectrometry;
Temperature;
Time Factors
- From:
Journal of Zhejiang University. Medical sciences
2020;49(5):565-573
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore effects of different delivery and storage conditions on concentrations of amino acids and carnitines in neonatal dried blood spots (DBS), so as to provide evidence for improving accurate and reliable detection by tandem mass spectrometry.
METHODS:A total of 1 254 616 newborn DBS samples in Newborn Screening Center of Zhejiang Province were delivered and stored at room temperature (group A,
RESULTS:The concentrations of amino acids and carnitines in the three groups were skewed, and the differences in amino acid and carnitine concentrations among groups were statistically significant (all
CONCLUSIONS:Cold-chain logistics system and storage in low temperature and low humidity can effectively reduce degradation of some amino acids and carnitines in DBS, improve the accuracy and reliability of detection, and thus ensures the quality of screening for neonatal metabolic diseases.
