Green tea extract as a cryoprotectant additive to preserve the motility and DNA integrity of human spermatozoa.

Author: Odai Am ALQAWASMEH ¹ ; Mingpeng ZHAO ¹ ; Carol Ps CHAN ¹ ; Maran Bw LEUNG ¹ ; Ki C CHOW ² ; Nikunj AGARWAL ² ; Jennifer Sm MAK ¹ ; Chi C WANG ¹ ; Chi P PANG ² ; Tin C LI ¹ ; Wai K CHU ² ; David Yl CHAN ¹
Author Information

1. Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
2. Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China.
Publication Type:Research Support, Non-U.S. Gov't
Keywords: antioxidants; cryopreservation additives; sperm DNA fragmentation; sperm cryopreservation; sperm motility
From: Asian Journal of Andrology 2021;23(2):150-156
CountryChina
Language:English
Abstract: Cryopreservation impairs sperm quality and functions, including motility and DNA integrity. Antioxidant additives in sperm freezing media have previously brought improvements in postthawed sperm quality. Green tea extract (GTE) is widely considered as an excellent antioxidant, and its beneficial role has been proven in other human cells. This study aims to evaluate the GTE as a potential additive in cryopreservation media of human spermatozoa. In part one, the semen of 20 normozoospermic men was used to optimize the concentration of GTE that maintains sperm motility and DNA integrity against oxidative stress, induced by hydrogen peroxide (H