Differential expression and bioinformatic analysis of microRNA in the plasma of patients with type 2 diabetes mellitus.
10.3760/cma.j.cn511374-20200103-00006
- Author:
Ying YE
1
;
Xiaohua LIU
;
Yansheng YAN
Author Information
1. Fujian Provincial Center for Disease Control, Fuzhou, Fujian 350001, China. yeying78@163.com.
- Publication Type:Journal Article
- MeSH:
Computational Biology;
Diabetes Mellitus, Type 2/genetics*;
Humans;
MicroRNAs/genetics*;
Phosphatidylinositol 3-Kinases;
Signal Transduction
- From:
Chinese Journal of Medical Genetics
2021;38(6):536-540
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct the differential expression profile of microRNA (miRNA) in plasma of patients with type 2 diabetes mellitus (T2DM) and explore the possibility of using miRNA as the target for diagnosis and treatment of T2DM.
METHODS:Agilent miRNA microarray was used to determine the expression profiles of miRNA in the plasma of patients with T2DM (FC> 2, P< 0.05). The result was verified by real-time quantitative PCR (RT-qPCR). Candidate miRNA was analyzed by bioinformatic tools.
RESULTS:In total 122 differentially expressed miRNAs were identified. Among these, 14 were selected by multi-source intersection screening, which included 5 up-regulated genes and 9 down regulated genes. RT-qPCR showed that the expression of hsa-miR-185-5p and hsa-miR-328-5p have significantly increased in T2DM patients (P< 0.05). Bioinformatic analysis suggested that these miRNAs may be involved in the pathogenesis of T2DM through insulin secretion and PI3K-AKT signaling pathway.
CONCLUSION:Differential expression of hsa-miR-185-5p and hsa-miR-328-5p in the plasma may be closely associated with the pathogenesis of T2DM.
