Genetic polymorphisms of 21 non-combined of DNA index system short tandem repeat loci in Hainan Li population.
10.3760/cma.j.cn511374-20201020-00735
- Author:
Tao LI
1
;
Yaqing ZHANG
;
Ying'ai ZHANG
Author Information
1. Hainan Huahai Forensic Institute of Judicial Expertise, Haikou, Hainan 570124, China. mwfandmwf@sina.com.
- Publication Type:Journal Article
- MeSH:
Asian Continental Ancestry Group/genetics*;
China;
DNA;
Gene Frequency;
Genetics, Population;
Humans;
Microsatellite Repeats/genetics*;
Polymorphism, Genetic
- From:
Chinese Journal of Medical Genetics
2021;38(5):503-505
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the genetic polymorphisms of 21 non-combined DNA index system short tandem repeat (STR) loci in Hainan Li population.
METHODS:DNA samples from 339 unrelated healthy individuals of Li population from Hainan Province were extracted and amplified with fluorescence labeled multiplex PCR system. PCR products were electrophoresed on an ABI3130 Genetic Analyzer following the manufacturer's instructions. Allele designation was performed with a GeneMapper ID-X by comparison with the allele ladder provided by the corresponding kit.
RESULTS:A total of 173 alleles and 489 genotypes were observed for the 21 STR loci, respectively. The frequencies of alleles and genotypes were 0.0010-0.5434 and 0.0020-0.3274, respectively. The heterozygosity varied from 0.639 to 0.833. Discrimination power (DP) was 0.803-0.948, power of exclusion for trio-paternity was 0.416-0.584, power of exclusion for duo-paternity was 0.140-0.238, the polymorphism information content(PIC) was 0.57-0.81, respectively. The total discrimination power (TDP), cumulative probability of exclusion for trio-paternity testing(CPE-trio) and cumulative probability of exclusion for duo-paternity testing (CPE-duo) were 0.999 999 999 999 99, 0.999 999 883 211 752, and 0.987 266, respectively.
CONCLUSION:The 21 STR loci are highly polymorphic and informative in the studied population and can be employed as supplementary loci in duo-paternity testing or cases with variant circumstances.
