Analysis of SCN1A gene variants among patients with Dravet syndrome.
10.3760/cma.j.cn511374-20200120-00041
- VernacularTitle:Dravet综合征患者
SCN1A基因变异分析
- Author:
Li LI
1
;
Dandan ZHU
Author Information
1. Department of Pediatrics, Luzhou Women and Children's Health Care Hospital, Luzhou, Sichuan 646000, China. dandanzhu1208@sina.com.
- Publication Type:Journal Article
- MeSH:
Epilepsies, Myoclonic/genetics*;
Genomics;
Humans;
Infant;
Mutation;
NAV1.1 Voltage-Gated Sodium Channel/genetics*;
Pedigree;
Spasms, Infantile/genetics*
- From:
Chinese Journal of Medical Genetics
2021;38(2):158-161
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the clinical features and genetic variants in two patients with Dravet syndrome (DS).
METHODS:Peripheral blood samples of the children and their parents were collected for the extraction of genomic DNA and high-throughput sequencing. Suspected variants were confirmed by Sanger sequencing.
RESULTS:By high-throughput sequencing, the two children were found to respectively harbor a c.2135delC frameshifting variant in exon 12 and a c.1522G>T nonsense variant in exon 10 of the SCN1A gene. Both variants were predicted to be pathogenic by bioinformatic analysis. Based on the American College of Medical Genetics and Genomics standards and guidelines, the c.2135delC and c.1522G>A variants of the SCN1A gene were predicted to be pathogenic (PVS1+ PS2+ PM2+ PP3).
CONCLUSION:The variants of the SCN1A gene probably underlay the DS in the patients. Above finding has enriched the variant spectrum and enabled genetic counseling for their families.
