Expression of Twist1, SIRT1, FGF2 and TGF-β3 genes and its regulatory effect on the proliferation of placenta, umbilical cord and dental pulp mesenchymal stem cells.
10.3760/cma.j.cn511374-20191217-00641
- Author:
Yao TAN
1
;
Yin DENG
;
Keyou PENG
;
Zhengzhou SUN
;
Jianqiu HUANG
;
Xuntong GU
;
Fusheng ZHANG
;
Hanqing PENG
;
Xuechao ZHANG
;
Rong ZHANG
Author Information
1. Chengdu Zhongchuang Qingke Medical Diagnose Test Center, Chengdu, Sichuan 610041, China. zr1223@126.com.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Cell Proliferation/genetics*;
Cells, Cultured;
Dental Pulp/cytology*;
Female;
Fibroblast Growth Factor 2/genetics*;
Humans;
Mesenchymal Stem Cells/cytology*;
Nuclear Proteins/genetics*;
Placenta/cytology*;
Pregnancy;
Sirtuin 1/genetics*;
Transforming Growth Factor beta3/genetics*;
Twist-Related Protein 1/genetics*;
Umbilical Cord/cytology*
- From:
Chinese Journal of Medical Genetics
2021;38(2):117-122
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare the mRNA level of cell proliferation-related genes Twist1, SIRT1, FGF2 and TGF-β3 in placenta mesenchymal stem cells (PA-MSCs), umbilical cord mensenchymals (UC-MSCs) and dental pulp mesenchymal stem cells (DP-MSCs).
METHODS:The morphology of various passages of PA-MSCs, UC-MSCs and DP-MSCs were observed by microscopy. Proliferation and promoting ability of the three cell lines were detected with the MTT method. Real-time PCR (RT-PCR) was used to determine the mRNA levels of Twist1, SIRT1, FGF2, TGF-β3.
RESULTS:The morphology of UC-MSCs and DP-MSCs was different from that of PA-MSCs. Proliferation ability and promoting ability of the PA-MSCs was superior to that of UC-MSCs and DP-MSCs. In PA-MSCs, expression level of Twist1 and TGF-β3 was the highest and FGF2 was the lowest. SIRT1 was highly expressed in UC-MSCs. With the cell subcultured, different expression levels of Twist1, SIRT1, FGF2, TGF-β3 was observed in PA-MSCs, UC-MSCs and DP-MSCs.
CONCLUSION:Up-regulated expression of the Twist1, SIRT1 and TGF-β3 genes can promote proliferation of PA-MSCs, UC-MSCs and DP-MSCs, whilst TGF-β3 may inhibit these. The regulatory effect of Twist1, SIRT1, FGF2 and TGF-β3 genes on PA-MSCs, UC-MSCs and DP-MSCs are different.
