Characterization of a pair of twins as blood group chimeras.
10.3760/cma.j.cn511374-20190913-00471
- Author:
Yuli ZHU
1
;
Wenhui MA
;
Haiyan WANG
;
Bin HU
;
Zhihui FENG
Author Information
1. Qingdao Blood Center, Qingdao, Shandong 266071, China. qd_hla@163.com.
- Publication Type:Journal Article
- MeSH:
ABO Blood-Group System/genetics*;
Alleles;
Chimera/genetics*;
Genotype;
Humans;
Twins/genetics*
- From:
Chinese Journal of Medical Genetics
2020;37(12):1399-1402
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To delineate the blood group for a pair of twins with inconclusive ABO blood typing result.
METHODS:Serological test for blood group was carried out by using ABO and Rh Blood Grouping Cards (Microcolumn Gel Immunoassay). Sequence specific primer-PCR (PCR-SSP), direct sequencing and TA clone sequencing were used to analyze the ABO gene. Genetic status was analyzed by using 16 short tandem repeat (STR) markers.
RESULTS:Red blood cells of the twins displayed 2+ mixed agglutination phenomenon with anti-A, anti-A1 and anti-E. PCR-SSP and DNA sequencing of exons 6 to 7 revealed that they have an ABO*O.01.01/ABO*O.01.02 genotype. DNA sequencing of microsatellite enhancer region revealed presence of A gene. STR analysis revealed more than two haplotypes for 9 loci between the twins. After clustered by anti-A, the red blood cells were divided into two groups: A, CcDEe and O, CcDee, respectively.
CONCLUSION:Serological and molecular techniques have characterized the twins as blood group chimeras.
