Expression and clinical significance of receptor-interacting protein serine-threonine kinases 1 in the nucleus pulposus of patients with lumbar disc herniation.
10.12200/j.issn.1003-0034.2021.04.014
- Author:
Mao-Cong WU
1
;
Ling-Ling WANG
1
;
Xin-Chang DENG
1
Author Information
1. Department of Spinal Surgery, Maoming People's Hospital, Southern Medical University, Maoming 525000, Guangdong, China.
- Publication Type:Journal Article
- Keywords:
Clinical significance;
Lumbar disc herniation;
Protein-serine-threonine kinases
- MeSH:
Humans;
Intervertebral Disc/metabolism*;
Intervertebral Disc Degeneration;
Intervertebral Disc Displacement/genetics*;
Nucleus Pulposus;
Receptor-Interacting Protein Serine-Threonine Kinases/genetics*;
Tumor Necrosis Factor-alpha/metabolism*
- From:
China Journal of Orthopaedics and Traumatology
2021;34(4):363-367
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression and clinical significance of receptor interacting protein serine-threonine kinases 1 (RIPK1) in the nucleus pulposus of patients with lumbar disc herniation (LDH).
METHODS:Nucleus pulposus tissue specimens of 40 patients with LDH patients underwent surgical treatment from January 2016 to January 2018 as the case group, and nucleus pulposus tissue specimens of 30 patients with lumbar spine fracture underwent surgical treatment at the same time as the control group. The expression of RIPK1 mRNA and protein of receptor interaction were detected by polymerase chain reaction (PCR) and Western blot, respectively. The expression of RIPK1 protein in the nucleus pulposus were detected by immunohistochemical staining. The concentrations of RIPK1 and tumor necrosis factor-α (TNF-α) in nucleus pulposus were detected by ELISA method. The relationship between the concentrations of RIPK1, TNF-α in nucleus pulposus and the Pearce grade of LDH patients was analyzed by one-way ANOVA. The correlation between RIPK1 and TNF-α was analyzed by Pearson.
RESULTS:RIPK1 was weakly positively expressed in nucleus pulposus of control group, and RIPK1 protein was positively or strongly positively expressed in case group. The expression of RIPK1 mRNA in nucleus pulposus of case group was higher than that of control group (
CONCLUSION:The expression levels of RIPK1 mRNA and protein in the intervertebral disc tissues of LDH patients are higher than those of normal intervertebral disc tissues, and increased with the increase of Pearce grade, which may be an important factor involved in LDH inflammatory disease.
