Rapid screening of single guide RNA targeting pig genome and the harvesting of monoclonal cells by microarray seal.
10.7507/1001-5515.202006032
- Author:
Mengyu GAO
1
,
2
;
Xinglong ZHU
3
,
4
,
5
;
Shisheng WANG
6
;
Bingqi ZHANG
3
,
4
,
5
;
Yunlin ZHANG
3
,
4
,
5
;
Yuting HE
3
,
4
,
5
;
Yanyan ZHOU
2
;
Shun LI
7
;
Guang YANG
8
;
Guangneng LIAO
8
;
Ji BAO
3
,
4
,
5
;
Hong BU
1
,
2
Author Information
1. Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China
2. Institute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
3. Institute of Clinical Pathology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China
4. Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC
5. West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
6. West China - Washington Mitochondria and Metabolism Research Center, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
7. Department of Biophysics, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, P.R.China.
8. Experimental Animal Center, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
- Publication Type:Journal Article
- Keywords:
fumarylacetoacetate hydrolase gene knockout;
gene editing for large animals;
patterned microarray;
rapid screening of sgRNA;
regular short repetitive Palindromic sequence clusters and CRISPR- associated proteins 9
- MeSH:
Animals;
CRISPR-Cas Systems/genetics*;
Clustered Regularly Interspaced Short Palindromic Repeats/genetics*;
Gene Editing;
RNA, Guide/genetics*;
Swine
- From:
Journal of Biomedical Engineering
2021;38(1):111-121
- CountryChina
- Language:Chinese
-
Abstract:
The emergence of regular short repetitive palindromic sequence clusters (CRISPR) and CRISPR- associated proteins 9 (Cas9) gene editing technology has greatly promoted the wide application of genetically modified pigs. Efficient single guide RNA (sgRNA) is the key to the success of gene editing using CRISPR/Cas9 technology. For large animals with a long reproductive cycle, such as pigs, it is necessary to screen out efficient sgRNA
