High throughput detection and characterization of red blood cells deformability by combining optical tweezers with microfluidic technique.
10.7507/1001-5515.201911020
- Author:
Meng ZHANG
1
;
Xiaochen MENG
1
;
Lianqing ZHU
1
Author Information
1. Beijing Key Laboratory for Optoelectronic Measurement Technology, Beijing Laboratory of Biomedical Testing Technology and Instruments, Beijing Information Science and Technology University, Beijing 100101, P.R.China.
- Publication Type:Journal Article
- Keywords:
high throughput detection;
microfluidic chip;
optical tweezer;
red blood cells deformability
- MeSH:
Erythrocyte Deformability;
Erythrocytes;
Humans;
Microfluidics;
Optical Tweezers;
Viscosity
- From:
Journal of Biomedical Engineering
2020;37(5):848-854
- CountryChina
- Language:Chinese
-
Abstract:
A high throughput measurement method of human red blood cells (RBCs) deformability combined with optical tweezers technology and the microfluidic chip was proposed to accurately characterize the deformability of RBCs statistically. Firstly, the effective stretching deformation of RBCs was realized by the interaction of photo-trapping force and fluid viscous resistance. Secondly, the characteristic parameters before and after the deformation of the single cell were extracted through the image processing method to obtain the deformation index of area and circumference. Finally, statistical analysis was performed, and the average deformation index parameters (
