Analysis and comparison of metabolic processes of salvianolic acid A and salvianolic acid B in rats based on UHPLC-LTQ-Orbitrap MS technology.
10.19540/j.cnki.cjcmm.20200820.201
- Author:
Bei-Bei MA
1
;
Tian-Yu LOU
1
;
Yao-Yue LIANG
1
;
Ting-Ting WANG
1
;
Rui-Ji LI
1
;
Jin-Hui LIU
1
;
Chen-Xiao WANG
1
;
Shang-Yue YU
1
;
Yu-Dong GUO
2
;
Jing WANG
1
;
Zhi-Bin WANG
3
Author Information
1. School of Chinese Pharmacy, Beijing University of Chinese Medicine Beijing 102488, China.
2. Beijing Institute for Drug Control Beijing 102200, China.
3. Tongrentang Technology Development Company Limited by Shares Beijing 100071, China.
- Publication Type:Journal Article
- Keywords:
UHPLC-LTQ-Orbitrap MS;
metabolites;
salvianolic acid A;
salvianolic acid B
- MeSH:
Animals;
Benzofurans;
Caffeic Acids;
Chromatography, High Pressure Liquid;
Lactates;
Mass Spectrometry;
Rats;
Technology
- From:
China Journal of Chinese Materia Medica
2021;46(9):2276-2286
- CountryChina
- Language:Chinese
-
Abstract:
The metabolites of salvianolic acid A and salvianolic acid B in rats were analyzed and compared by ultra-high-perfor-mance liquid chromatography with linear ion trap-orbitrap mass spectrometry(UHPLC-LTQ-Orbitrap MS). After the rats were administrated by gavage, plasma at different time points and urine within 24 hours were collected to be treated by solid phase extraction(SPE), then they were gradient eluted by Acquity UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm) and 0.1% formic acid solution(A)-acetonitrile(B) mobile phase system, and finally all biological samples of rats were analyzed under negative ion scanning mode. By obtaining the accurate relative molecular mass and multi-level mass spectrometry information of metabolites, combined with the characteristic cleavage law of the reference standard and literature reports, a total of 30 metabolites, including salvianolic acid A and B, were identified. Among them, there were 24 metabolites derived from salvianolic acid A, with the main metabolic pathways including ester bond cleavage, dehydroxylation, decarboxylation, hydrogenation, methylation, hydroxylation, sulfonation, glucuronidation, and their multiple reactions. There were 15 metabolites of salvianolic acid B, and the main biotransformation pathways were five-membered ring cracking, ester bond cleavage, decarboxylation, dehydroxylation, hydrogenation, methylation, sulfonation, glucuronidation, and their compound reactions. In this study, the cross-metabolic profile of salvianolic acid A and B was elucidated completely, which would provide reference for further studies on the basis of pharmacodynamic substances and the exploration of pharmacological mechanism.
