Content determination of five flavonoids in Tibetan medicine Rhododendron anthopogonoides by quantitative analysis of multi-components by single marker (QAMS).
10.19540/j.cnki.cjcmm.20210129.302
- Author:
Yao PENG
1
;
Rong ZENG
1
;
Shang-Jie WU
2
;
Si LEI
2
;
Zhi-Na SUN
3
;
Xiu-Qin HE
3
;
Juan LI
1
;
Shun-Xiang LI
1
Author Information
1. Hunan Province Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, School of Pharmacy,Hunan University of Chinese Medicine Changsha 410208, China.
2. the Second Xiangya Hospital of Central South University Changsha 410008, China.
3. Qinghai Red Cross Hospital Xining 810000, China.
- Publication Type:Journal Article
- Keywords:
Rhododendron anthopogonoides;
hyperoside;
quantitative analysis of multi-components by single marker(QAMS);
relative correction factor;
rutin
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Flavonoids;
Medicine, Tibetan Traditional;
Reproducibility of Results;
Rhododendron
- From:
China Journal of Chinese Materia Medica
2021;46(9):2229-2236
- CountryChina
- Language:Chinese
-
Abstract:
To establish a quantitative analysis of multi-components by single marker(QAMS) method for five flavonoids in Rhododendron anthopogonoides and verify its feasibility and applicability in the medicinal materials of R. anthopogonoides. With hyperoside as the internal reference, relative correction factors(RCF) of rutin, quercetin, quercitrin and kaempferol were established by high-performance liquid chromatography(HPLC) analysis. RCFs were used to calculate the content of each component, system durability and relative retention time. Simultaneously, QAMS and external standard method(ESM) were used to determine the content of five flavonoids in 12 batches of R. anthopogonoides from different origins. The results were statistically analyzed to verify the accuracy and feasibility. The fingerprints and cluster analysis data of R. anthopogonoides analyzed and discussed differences among the batches. According to the results, the RCFs of rutin, quercetin, quercetin and kaempferol in R. anthopogonoides were 1.242 6, 0.990 5, 0.535 0, and 0.781 3, respectively. The RCFs represented a good reproducibility under different experimental conditions. Besides, there was no significant difference between QAMS and ESM. Besides, the fingerprint and cluster analysis data showed the consistency between the classification and with the origin distribution of the herbs. In conclusion, the QAMS method shows a good stability and accuracy in the quality control of R. anthopogonoides.
