Effect of self-assembled nanoparticles from Shaoyao Gancao Decoction on release and absorption of main components of Baishao.
10.19540/j.cnki.cjcmm.20210222.301
- Author:
Cheng-Ying SHEN
1
;
Jun-Jun ZHU
1
;
Bo DAI
2
;
Xiao-Fang LI
3
;
Bao-de SHEN
2
;
Hai-Long YUAN
2
Author Information
1. Department of Pharmacy,Air Force Medical Center Beijing 100142, China School of Pharmacy,Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
2. Department of Pharmacy,Air Force Medical Center Beijing 100142, China.
3. School of Pharmacy,Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
- Publication Type:Journal Article
- Keywords:
Shaoyao Gancao Decoction;
encapsulation;
in vitro release;
intestinal absorption;
morphology;
particle size;
self-assembled nanoparticles
- MeSH:
Drugs, Chinese Herbal;
Intestinal Absorption;
Intestines;
Nanoparticles
- From:
China Journal of Chinese Materia Medica
2021;46(9):2190-2196
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of self-assembled nanoparticles from Shaoyao Gancao Decoction(SGD-SAN) on the encapsulation, in vitro release and intestinal absorption of the main components of Baishao. Particle size analysis and morphological observation were used to verify the formation of SGD-SAN in the decoction. The entrapment efficiency(EE) of SGD-SAN on the main components of Baishao was determined by ultrafiltration centrifugation. The dialysis bag method was used to study the in vitro release of the main components of Baishao with pH 6.8 phosphate buffer solution as the release media. Single-pass intestinal perfusion study was performed to investigate the effect of SGD-SAN on the absorption of the main components of Baishao. The results showed that there were nanoparticles in the SGD, and the particle sizes and PDI of SGD-SAN were about 200 nm and 0.38, respectively. SGD-SAN was irregularly spherical under transmission electron microscope(TEM). The EEs of albiflorin, paeoniflorin and benzoylpaeoniflorin in SGD-SAN were 33.78%±1.03%,33.61%±0.90%,88.53%±0.58%, respectively. The release characteristics of albiflorin, paeoniflorin and benzoylpaeoniflorin from SGD-SAN showed a slow-release effect on pH 6.8 phosphate buffer solution media. SGD-SAN could significantly enhance the absorption of albiflorin, paeoniflorin and benzoylpaeoniflorin in the ileum. The results of this study indicated that SAN could be formed during the mixed decoction of Baishao and Gancao, and SGD-SAN could encapsulate the components of Baishao, with a certain slow-release effect, and the formation of SAN facilitated the absorption of drugs in the ileum.
