Effects of drought stress on physiological and biochemical and chemical components of Cinnamomum cassia seedlings.
10.19540/j.cnki.cjcmm.20210224.101
- Author:
Lei ZHONG
1
;
Pei-Ran LIAO
1
;
Chang-Zheng LIU
1
;
Jia-Ping QIAN
1
;
Wan-Cong HE
1
;
Bi LUO
1
;
Quan YANG
1
Author Information
1. Guangdong Provincial Southern Medicine Standardized Cultivation and Comprehensive Development Engineering Technology Research Center, Guangzhou Comprehensive Test Station of National Traditional Chinese Medicine Industry Technology System, the Key Laboratory of Lingnan Medicinal Materials Production and Development of the State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China.
- Publication Type:Journal Article
- Keywords:
Cinnamomum cassia;
chemical composition;
drought stress;
physiological and biochemical
- MeSH:
Catalase;
Cinnamomum aromaticum;
Droughts;
Malondialdehyde;
Seedlings;
Stress, Physiological;
Superoxide Dismutase
- From:
China Journal of Chinese Materia Medica
2021;46(9):2158-2166
- CountryChina
- Language:Chinese
-
Abstract:
Six month old Cinnamomum cassia seedlings were used to simulate drought stress with polyethylene glycol(PEG 6000). The physiological indicators(osmotic substances, antioxidant enzymes, etc.) and chemical components of seedlings under different drought levels and the correlation between the two were studied. The results showed that the chlorophyll content and relative water content decreased gradually with the increase of PGE 6000(0, 5%, 10%, 15%) concentration and time(3, 5, 7 d), while the soluble protein content, soluble sugar content and catalase(CAT) activity increased, but the rising rate slowed down with the time. The activities of peroxidase(POD), superoxide dismutase(SOD), malondialdehyde(MDA) and proline content increased at first and then decreased. The content of coumarin, cinnamaldehyde, cinnamic acid and dimethoxycinnamaldehyde decreased, while the content of cinnamyl alcohol continued to increase.Under drought stress, the fluorescence signals of reactive oxygen species and no contents in roots of C. cassia seedlings were significantly stronger than those of the control.Further correlation analysis showed that coumarin content, di-methoxycinnamaldehyde content and osmoregulation substance content were significantly negatively correlated(P<0.05), cinnamic acid content was significantly negatively correlated with POD and SOD activities(P<0.01).It was found that C. cassia seedlings showed a certain degree of drought tolerance under short-term or mild drought stress, but if the drought exceeded a certain degree, the physiological metabolism of the seedlings would be unbalanced.
