Simultaneous determination of multiple bioactive constituents in Abelmoschi Corolla by UFLC-QTRAP-MS/MS.
10.19540/j.cnki.cjcmm.20201225.302
- Author:
Sheng-Xin YIN
1
;
Li-Fang WEI
1
;
Yu-Qi MEI
1
;
Xun-Hong LIU
1
;
Li-Si ZOU
1
;
Zhi-Chen CAI
1
;
Jia-Huan YUAN
1
;
Hai-Tao GE
2
;
Dian-Guang WANG
2
;
Dan-Dan WANG
2
Author Information
1. School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
2. Jiangsu Suzhong Pharmaceutical Group Co., Ltd. Taizhou 225500, China.
- Publication Type:Journal Article
- Keywords:
Abelmoschi Corolla;
UFLC-QTRAP-MS/MS;
bioactive constituents;
gray relational analysis;
harvesting and processing
- MeSH:
Amino Acids;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Nucleosides;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(10):2527-2536
- CountryChina
- Language:Chinese
-
Abstract:
A comprehensive analytical method based on ultra-fast liquid chromatography coupled with triple quadrupole/linear ion trap tandem mass spectrometry(UFLC-QTRAP-MS/MS) was established for simultaneous determination of the content of 38 active components in Abelmoschi Corolla, including flavonoids, organic acids, nucleosides and amino acids, so as to investigate the effects of different harvesting and processing methods on multi-active components in Abelmoschi Corolla. The chromatographic separation was performed on a XBridg®C_(18) column(4.6 mm×100 mm, 3.5 μm) with(0.1% formic acid water) methanol-acetonitrile(1∶1) as the mobile phase for gradient elution at 30 ℃. The flow rate was 0.5 mL·min~(-1). The components were detected in a multiple-reaction monitoring(MRM) mode. The gray relational analysis(GRA) was used to comprehensively evaluate the multiple active components of Abelmoschi Corolla at different harvesting times and drying temperatures. The results showed that 38 components had a good linearity with correlation coefficients all above 0.999 0. The method featured a good precision, repeatability and stability with the relative stan-dard deviations(RSDs) of less than 5.0%. Recoveries ranged from 98.06% to 104.4% with RSD between 0.22% and 4.9%. The results of GRA indicated that a better quality in the samples collected on September 9 th. Samples dried at 90 ℃ had a better quality. The established method is accurate and reliable, and can be used to assess the internal quality of Abelmoschi Corolla. This study can provide basic materials for determining appropriate harvesting time and processing method of Abelmoschi Corolla.
