Chemical constituents from Morinda citrifolia and their inhibitory activities on proliferation of synoviocytes in vitro.
10.19540/j.cnki.cjcmm.20210224.601
- Author:
Ying-Ying ZHAO
1
;
Qiao-Mei YU
2
;
Ze-Hua QIAO
2
;
Juan LI
2
;
Hao-Xuan TANG
2
;
Guang-Ying CHEN
1
;
Yan-Hui FU
1
Author Information
1. China School of Pharmacy, Fujian University of Traditional Chinese Medicine Fuzhou 350122, China Engineering Research Center for Industrialization of Southern Medicinal Plants Resources of Hainan Province,Hainan Normal University Haikou 571158, China Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University Haikou 571158, China.
2. Engineering Research Center for Industrialization of Southern Medicinal Plants Resources of Hainan Province,Hainan Normal University Haikou 571158, China Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University Haikou 571158, China.
- Publication Type:Journal Article
- Keywords:
Morinda citrifolia;
fatty acids;
inhibitory activities on proliferation of synoviocytes;
lignans;
sesquiterpenes;
steroids
- MeSH:
Cell Proliferation;
Chromatography, High Pressure Liquid;
Molecular Structure;
Morinda;
Synoviocytes
- From:
China Journal of Chinese Materia Medica
2021;46(10):2519-2526
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents from the stems and leaves of Morinda citrifolia were isolated and purified by column chromatography methods with silica gel, ODS, Sephadex LH-20 and preparative high performance liquid chromatography(HPLC). The structures of the isolated compounds were identified by physicochemical properties and spectroscopic analysis, as well as comparisons with the data reported in literature. 17 compounds were isolated from the 90% ethanol extract of the stems and leaves of M. citrifolia, and were identified as 9,10-dihydroxy-4, 7-megastigmadien-3-one(1), 5,12-epoxy-6,9-hydroxy-7-megastigmen-3-one(2), fukinone(3), β-eudesmol(4), sarmentol F(5), 4, 5-dihydroblumenol A(6), 3-hydroxy-β-ionone(7), aristol-8-en-1-one(8), ergosta-7-en-3β-ol(9), ergosta-7-ene-3β,5α,6β-triol(10),(22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(11), olivil(12), 4-epi-larreatricin(13), chushizisin Ⅰ(14), rabdosia acid A(15), glycerol monolinoleate(16) and(9Z,12Z,15Z)-2,3-dihydroxypropyl octadeca-trienoate(17). All compounds were isolated from M. citrifolia for the first time. All isolated compounds were evaluated for their anti-rheumatoid arthritis activities via examining their inhibitory activities on the proliferation of synoviocytes in vitro using MTS met-hod. Compounds 1-11 showed significant anti-rheumatoid arthritis activities, displaying the inhibitory effects on the proliferation of MH7 A synovial fibroblast cell with the IC_(50) values ranging from(38.69±0.86) to(203.45±1.03) μmol·L~(-1).
