Application prospects of single-cell transcriptome sequencing in traditional Chinese medicine research.
10.19540/j.cnki.cjcmm.20210218.601
- Author:
Ju-Qin PENG
1
;
Jun-Guo REN
1
;
Jian-Xun LIU
1
Author Information
1. Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Institute of Basic Medical Sciences, Xiyuan Hospital,China Academy of Chinese Medical Sciences Beijing 100091, China.
- Publication Type:Journal Article
- Keywords:
cell heterogeneity;
drug research;
single-cell transcriptome sequencing;
traditional Chinese medicine
- MeSH:
Gene Expression Profiling;
Medicine, Chinese Traditional;
Reference Standards;
Sequence Analysis, RNA;
Single-Cell Analysis;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2021;46(10):2456-2460
- CountryChina
- Language:Chinese
-
Abstract:
Single-cell transcriptome sequencing(scRNA-seq) can be used to analyze the expression characteristics of the transcriptome at the level of individual cell, and discover the heterogeneity of gene expression in individual cell that is "diluted" or averaged in study of group organization. The scRNA-seq, with the characteristics of standardization, high-throughput, and high integration, can greatly simplify the experimental operation and significantly reduce the consumption of reagents. At the same time, a variety of cells are screened and the gene expression patterns are analyzed at the single-cell level to provide a more efficient detection technique and more rich and accurate information for drug research. In the field of traditional Chinese medicine(TCM), the scRNA-seq is still a new technology, but the individual and precision concepts embodied by scRNA-seq and the theory of TCM syndrome differentiation and treatment have reached the same effect between the micro and macro aspects. This study tried to broaden the thinking for the modernization of TCM by introducing the development of scRNA-seq technology and its application in modern drug research and discussing the application prospects of scRNA-seq in TCM research.
