Analysis of water-soluble nutrients in Lycium barbarum leaves and differences between different producing areas.
10.19540/j.cnki.cjcmm.20200701.201
- Author:
Xue-Qin ZHAO
1
;
Sheng GUO
1
;
You-Yuan LU
1
;
Fang ZHANG
1
;
Hui YAN
1
;
Han-Qing WANG
2
;
Jin-Ao DUAN
1
Author Information
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, Nanjing University of Chinese Medicine Nanjing 210023, China.
2. School of Pharmacy, Ningxia Medical University Yinchuan 750021, China.
- Publication Type:Journal Article
- Keywords:
Lycium barbarum;
Lycium barbarum leaves;
differences of production area;
nutritional components;
principal component analysis
- MeSH:
Chromatography, Liquid;
Lycium;
Nutrients;
Plant Leaves;
Tandem Mass Spectrometry;
Water
- From:
China Journal of Chinese Materia Medica
2021;46(8):2084-2093
- CountryChina
- Language:Chinese
-
Abstract:
This study is to clarify the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas. The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS. The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively. Besides, eight nucleosides and twelve amino acids were detected in LBLs, and their average total contents were 54.95, 336.9 μg·g~(-1). Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine. In this study, the compositions and contents of water-soluble nutrients in LBLs were preliminarily clarified, which provided basis for further development and utilization of LBLs resoures.
