Research strategies for endophytes in medicinal plants based on high-throughput sequencing and traditional culture and isolation methods.
10.19540/j.cnki.cjcmm.20210127.101
- Author:
Hong-Yang WANG
1
;
Chuan-Zhi KANG
2
;
Sheng WANG
2
;
Dai-Quan JIANG
2
;
Zheng PENG
1
;
Yang XU
1
;
Yong-Xi DU
3
;
Yan ZHANG
2
;
Da-Hui LIU
4
;
Lan-Ping GUO
2
Author Information
1. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Hubei University of Chinese Medicine Wuhan 430065, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Guangdong Pharmaceutical University Guangzhou 510006, China.
4. Hubei University of Chinese Medicine Wuhan 430065, China.
- Publication Type:Journal Article
- Keywords:
endophytes;
high-throughput sequencing technology;
medicinal plants;
traditional culture and isolation methods
- MeSH:
Endophytes/genetics*;
High-Throughput Nucleotide Sequencing;
Plants, Medicinal;
Research Design
- From:
China Journal of Chinese Materia Medica
2021;46(8):1910-1919
- CountryChina
- Language:Chinese
-
Abstract:
The research on endophytes of medicinal plants mainly relies on the traditional culture and isolation methods. Because of their functions such as promoting host growth, improving stress resistance, promoting the accumulation of medicinal active ingredients or directly producing medicinal active ingredients, the endophytes of medicinal plants have gradually attracted wide attention. However, it was found that the strains isolated by traditional methods were not the true dominant endophytes of medicinal plants by comparing the results of traditional culture isolation with high-throughput sequencing. The blind and random nature of traditional methods leads to the lack of standards in terms of medium selection, culture time and interaction between species. On the contrary, high-throughput sequencing technology is an emerging molecular biology technology developed in recent decades. Due to its high resolution level and indepen-dent culture, it can be used for thorough analysis of the community structure and diversity of environmental microorganisms. Therefore, we proposed the strategy of using high-throughput sequencing technology to guide the traditional culture and isolation of endophytes from medicinal plants. Firstly, the endophytic structure and diversity of medicinal plants were completely clear by high-throughput sequencing technology, and the dominant endophytes of the host were unequivocal. Then according to the characteristics of each dominant endophytes design or query suitable medium for its growth to culture and isolation. Finally, the function of the isolates was studied. This method can prevent researchers from missing out on the important functional strains of the host, expand the research scope of endophytes of medicinal plants, and facilitate the in-depth excavation and utilization of endophytes of medicinal plants.
