Involvement of Transglutaminase-2 in alpha-MSH-Induced Melanogenesis in SK-MEL-2 Human Melanoma Cells.
- Author:
Hyun Ji KIM
1
;
Hye Ja LEE
;
Mi Kyung PARK
;
Kyung Jin GANG
;
Hyun Jung BYUN
;
Jeong Ho PARK
;
Mi Kyung KIM
;
Soo Youl KIM
;
Chang Hoon LEE
Author Information
1. BK21PLUS R-FIND team, College of Pharmacy, Dongguk University, Seoul 100-715, Republic of Korea. uatheone@dongguk.edu
- Publication Type:Original Article
- Keywords:
Cystamine;
Melanogenesis;
Transglutaminase-2;
TRP-1;
TRP-2;
SK-MEL-2 melanoma cells
- MeSH:
Blotting, Western;
Cystamine;
Dendrites;
Enzyme-Linked Immunosorbent Assay;
Gene Silencing;
Humans;
Hyperpigmentation;
Melanins;
Melanoma*;
Skin
- From:Biomolecules & Therapeutics
2014;22(3):207-212
- CountryRepublic of Korea
- Language:English
-
Abstract:
Skin hyperpigmentation is one of the most common skin disorders caused by abnormal melanogenesis. The mechanism and key factors at play are not fully understood. Previous reports have indicated that cystamine (CTM) inhibits melanin synthesis, though its molecular mechanism in melanogenesis remains unclear. In the present study, we investigated the effect of CTM on melanin production using ELISA reader and the expression of proteins involved in melanogenesis by Western blotting, and examined the involvement of transglutaminase-2 (Tgase-2) in SK-MEL-2 human melanoma cells by gene silencing. In the results, CTM dose-dependently suppressed melanin production and dendrite extension in alpha-MSH-induced melanogenesis of SK-MEL-2 human melanoma cells. CTM also suppressed alpha-MSH-induced chemotactic migration as well as the expressions of melanogenesis factors TRP-1, TRP-2 and MITF in alpha-MSH-treated SK-MEL-2 cells. Meanwhile, gene silencing of Tgase-2 suppressed dendrite extension and the expressions of TRP-1 and TRP-2 in alpha-MSH-treated SK-MEL-2 cells. Overall, these findings suggested that CTM suppresses alpha-MSH-induced melanogenesis via Tgase-2 inhibition and that therefore, Tgase-2 might be a new target in hyperpigmentation disorder therapy.
