Critical quality attribute assessment of big brand traditional Chinese medicine: modular identification of Tongren Niuhuang Qingxin Pills based on efficacy with chemical properties.
10.19540/j.cnki.cjcmm.20210218.301
- Author:
Le-Ting LEI
1
;
Li-Juan MA
1
;
Yu-Nan WEI
1
;
Ling LIN
1
;
Jin-Yuan ZHU
1
;
Jing-Qi ZENG
1
;
Jing ZHANG
1
;
Qian-Qian LI
1
;
Lu YAO
2
;
Zhi-Sheng WU
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Engineering Research Center of Chinese Medicine Production and New Drug Development, Ministry of Education Beijing 102488,China.
2. National Engineering Research Center for R&D of TCM Multi-ingredient Drugs, Institute of Science,Beijing Tongrentang Co., Ltd. Beijing 100079, China.
- Publication Type:Journal Article
- Keywords:
Tongren Niuhuang Qingxin Pills(TRNHQXP);
big brand traditional Chinese medicine(TCM);
critical quality attribute(CQA);
efficacy;
network pharmacology
- MeSH:
Chromatography, Liquid;
Drugs, Chinese Herbal;
Medicine, Chinese Traditional;
Quality Control
- From:
China Journal of Chinese Materia Medica
2021;46(7):1606-1615
- CountryChina
- Language:Chinese
-
Abstract:
Identification of critical quality attribute(CQA) is crucial in quality control of Tongren Niuhuang Qingxin Pills(TRNHQXP). In this study, 661 active components in TRNHQXP were selected by liquid chromatography-mass spectrometry(LC-MS) and network pharmacology based on reported data and TCMSP, BATMAN-TCM, and TCMID databases, as well as mass spectrometry data, and 1 413 targets of the active components were obtained through SwissTargetPrediction. The 152 potential targets obtained from the intersection of predicted targets with 456 stroke targets underwent functional enrichment analysis by Metascape. The 27 Chinese medicinals in TRNHQXP were divided into four sets according to efficacies. Thirty-seven key targets in the blood-activating and stasis-resolving set and 41 in the tonifying set were screened out. On the basis of these potential key targets, 137 potential key CQA of TRNHQXP for stroke were reversely predicted. This study revealed the possible mechanism of TRNHQXP in treating stroke and established a modular identification method for the potential CQA of big brand traditional Chinese medicine(TCM) based on efficacies and chemical properties. Consequently, the CQA of TRNHQXP were identified by this method, which has provided a reference for the following experimental studies of CQA.
