Protective effect of ginsenoside Rg_1 on hypoxia/reoxygenation injury and its mechanism.
10.19540/j.cnki.cjcmm.20201123.401
- Author:
Qian-Hui LI
1
;
Zhuo-Wang GE
2
;
Ding TIAN
2
;
Yin XIANG
2
;
Yu CHEN
3
;
Ya-Chen ZHANG
2
Author Information
1. Department of Geriatrics,Zhongda Hospital,Southeast University Nanjing 210009,China Department of Cardiology,Xinhua Hospital,School of Medicine,Shanghai Jiaotong University Shanghai 200092,China.
2. Department of Cardiology,Xinhua Hospital,School of Medicine,Shanghai Jiaotong University Shanghai 200092,China.
3. Department of Cardiology,Sixth People's Hospital,Shanghai Jiaotong University Shanghai 200233,China.
- Publication Type:Journal Article
- Keywords:
apoptosis;
ginsenoside Rg_1;
hypoxia/reoxygenation;
ischemia-reperfusion injury
- MeSH:
Apoptosis;
Ginsenosides/pharmacology*;
Heme Oxygenase-1/genetics*;
Humans;
Hypoxia;
Myocytes, Cardiac;
NF-E2-Related Factor 2/genetics*
- From:
China Journal of Chinese Materia Medica
2021;46(6):1460-1466
- CountryChina
- Language:Chinese
-
Abstract:
This project aimed to explore the protective effect of ginsenoside Rg_1 on hypoxia/reoxygenation(H/R)-induced H9 c2 cardiomyocyte injury and its underlying signaling pathway. The H/R model of H9 c2 cardiomyocytes was established and then the cells were divided into different treatment groups. CCK-8(cell counting kit-8) was used to detect the activity of cardiomyocytes; Brdu assay was used to detect the proliferation of H9 c2 cells; the caspase-3 activity was tested, and then the protein expression was assessed by Western blot. Flow cytometry was used to evaluate the apoptosis level of cardiomyocytes. Ginsenoside Rg_1 inhibited H/R-induced cardiomyocyte apoptosis and caspase-3 activity, promoted nuclear transcription of nuclear factor erythroid-2 related factor 2(Nrf2), and enhanced the expression of the downstream heme oxygenase-1(HO-1). Ginsenoside Rg_1 could increase Nrf2 nuclear transcription and HO-1 expression with the increase of concentration(10, 20, 40, 60 μmol·L~(-1)). However, the protective effect of ginsenoside Rg_1 on cardiomyocytes was significantly weakened after the transfection of Nrf2-siRNA. Ginsenoside Rg_1 could protect cardiomyocytes by activating the Nrf2/HO-1 pathway.
