Main chemical constituents in aerial parts of Glycyrrhiza uralensis by UPLC-Q-Exactive Orbitrap-MS.
10.19540/j.cnki.cjcmm.20201225.301
- Author:
Guan-Hua CHANG
1
;
Ying-Yi BO
2
;
Jie CUI
3
;
Lu-Lu XU
4
;
Zi-Han ZHAO
1
;
Wen-Quan WANG
5
;
Jun-Ling HOU
6
Author Information
1. School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488,China.
2. School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488,China Pharmacy Department,Tianjin Baodi District People's Hospital Tianjin 301800,China.
3. Institute of Medicinal Plant Development,Chinese Academy of Medicinal Sciences and Peking Union Medical Collage Beijing 100193,China.
4. School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488,China State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University Beijing 100191,China.
5. School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488,China Institute of Medicinal Plant Development,Chinese Academy of Medicinal Sciences and Peking Union Medical Collage Beijing 100193,China Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs,Ministry of Education Beijing 102488,China.
6. School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 102488,China Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs,Ministry of Education Beijing 102488,China.
- Publication Type:Journal Article
- Keywords:
UPLC-Q-Exactive Orbitrap-MS;
aerial parts of Glycyrrhiza uralensis;
chemical constituents;
flavonoids;
stilbenes
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Glycyrrhiza uralensis;
Mass Spectrometry;
Plant Components, Aerial
- From:
China Journal of Chinese Materia Medica
2021;46(6):1449-1459
- CountryChina
- Language:Chinese
-
Abstract:
Chemical constituents from aerial parts of Glycyrrhiza uralensis were analyzed and identified using ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS). The chromatographic column of Waters Acquity UPLC BEH-C_(18)(2.1 mm×100 mm, 1.7 μm) was adopted, with acetonitrile-water(0.5% formic acid) as mobile phase at a flow rate of 0.2 mL·min~(-1). Data was collected in positive and negative modes of electrospray ionization(ESI). A total of 55 compounds, including 42 flavonoids, 9 stilbenes, 2 coumarins, 1 lignin and 1 phenolic acid, which were characterized in the aerial parts of G. uralensis based on accurate molecular mass information of molecular and product ions provided by UPLC-Q-Exactive Orbitrap-MS based on comparison with standard substances and references. It is an effective and accurate method to provide chemical information of constituents in aerial parts of G. uralensis, and can provide a reference for further study on pharmacodynamic material basis and resources development and utilization.
