Analysis of flavonoids from saffron floral bio-residues.
10.19540/j.cnki.cjcmm.20201214.302
- Author:
Jun-Jie XIANG
1
;
Na CHEN
2
;
Hua LI
2
;
Xue ZHANG
3
;
Bin YANG
2
;
Lu-Qi HUANG
4
Author Information
1. Academician Workstation of Jiangxi University of Traditional Chinese Medicine Nanchang 330004,China Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
2. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
3. Shanghai Traditional Chinese Medicine Co.,Ltd. Shanghai 200003,China.
4. Academician Workstation of Jiangxi University of Traditional Chinese Medicine Nanchang 330004,China China Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
UPLC-Q-TOF-MS qualitative analysis;
content determination;
fingerprint;
flavonoids;
saffron floral bio-residues
- MeSH:
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Crocus;
Flavonoids;
Flowers;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(6):1438-1449
- CountryChina
- Language:Chinese
-
Abstract:
In order to better utilize saffron floral bio-residues(SFB), a qualitative and quantitative analysis of flavonoids in SFB was conducted using UPLC-MS and UPLC, respectively. On the one hand, 50 flavonols and 5 anthocyanins were putatively characte-rized by using UPLC-Q-TOF-MS. On the other hand, an UPLC method was established for determining the fingerprint of SFB as well as testing the main flavonoids kaempferol-3-O-sophoroside and delphinidin-3,5-di-O-glucoside. Contents of kaempferol-3-O-sophoroside and delphinidin-3,5-di-O-glucoside of 10 batches of samples were 44.21-58.73 mg·g~(-1) and 2.11-6.37 mg·g~(-1), respectively, and the similarities of 10 batches were more than 0.99. In addition, the color of the samples was digitized by using electronic eye technology, and it was found that the color of the samples was significantly correlated with the content of delphinidin-3,5-di-O-glucoside. The richness of flavonoids in SFB indicated its potential for development and utilization, and the large variation in anthocyanin content among samples from different regions suggested that more attention should be paid to the methods of sample pretreatment and storage.
