Luteolin Inhibits the Activity, Secretion and Gene Expression of MMP-3 in Cultured Articular Chondrocytes and Production of MMP-3 in the Rat Knee.
- Author:
Bun Jung KANG
1
;
Jiho RYU
;
Choong Jae LEE
;
Sun Chul HWANG
Author Information
1. Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-702, Republic of Korea. hscspine@hanmail.net
- Publication Type:Original Article
- Keywords:
Osteoarthritis;
Luteolin;
Chondrocyte
- MeSH:
Animals;
Blotting, Western;
Caseins;
Chondrocytes*;
Collagen;
Collagen Type II;
Gene Expression*;
Interleukin-1beta;
Knee*;
Luteolin*;
Osteoarthritis;
Polymerase Chain Reaction;
Rats*;
Reverse Transcription;
Thrombospondins
- From:Biomolecules & Therapeutics
2014;22(3):239-245
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated whether luteolin affects the gene expression, secretion and activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as production of MMP-3 in the rat knee to evaluate the potential chondroprotective effects of luteolin. Rabbit articular chondrocytes were cultured in a monolayer and IL-1beta-induced gene expression levels of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5 and type II collagen were measured by reverse transcription - polymerase chain reaction (RT-PCR). Effects of luteolin on interleukin-1beta (IL-1beta)-induced secretion and enzyme activity of MMP-3 in rabbit articular chondrocytes were investigated by western blot analysis and casein zymography, respectively. The effect of luteolin on MMP-3 protein production was also examined in vivo. The results were as follows: (1) luteolin inhibited the gene expression levels of MMP-3, MMP-1, MMP-13, ADAMTS-4 and ADAMTS-5. However, it increased the gene expression level of collagen in rabbit articular chondrocytes; (2) luteolin inhibited the secretion and activity of MMP-3; (3) luteolin inhibited in vivo production of MMP-3 protein. These results suggest that luteolin can regulate the gene expression, secretion and activity of MMP-3, by directly acting on articular chondrocytes.
