Effect of Huangqin Decoction on pyroptosis pathway of NLRP3/caspase-1 in mice with ulcerative colitis.
10.19540/j.cnki.cjcmm.20201117.402
- Author:
Na WU
1
;
Zhi-Ping WAN
2
;
Ling HAN
3
;
Hui-Ying LIU
4
;
Hua-Shan LI
5
Author Information
1. Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine Nanchang 330006,China.
2. Nanchang Hongdu Hospital of Traditional Chinese Medicine Nanchang 330000,China.
3. Yichun University Yichun 336000,China.
4. Jiangzhong Pharmaceutical Group Co.,Ltd. Nanchang 330004,China.
5. Guang'anmen Hospital,China Academy of Chinese Medical Sciences Beijing 100053,China.
- Publication Type:Journal Article
- Keywords:
Huangqin Decoction;
NLRP3/caspase-1 signaling pathway;
pyroptosis;
ulcerative colitis
- MeSH:
Animals;
Caspase 1/genetics*;
Colitis, Ulcerative/drug therapy*;
Mice;
NLR Family, Pyrin Domain-Containing 3 Protein/genetics*;
Pyroptosis;
Scutellaria baicalensis
- From:
China Journal of Chinese Materia Medica
2021;46(5):1191-1196
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effect of Huangqin Decoction on ulcerative colitis(UC) pyroptosis, and to explain the mechanism of pyroptosis based on NOD-like receptor thermoprotein domain 3(NLRP3)/cysteine proteinase 1(caspase-1) pathway. The animal model of UC induced with 3% dextran sodium sulfate(DSS) was established. The experimental animals were divided into control group, model group, low-dose(4.55 g·kg~(-1)), medium-dose(9.1 g·kg~(-1)) and high-dose(18.2 g·kg~(-1)) Huangqin Decoction groups and salazosulfapyridine group(0.45 g·kg~(-1)). While modeling, intragastric administration was given for 7 consecutive days. On the 8 th day, the mice were euthanized, the colon length was collected, and the histopathological changes were observed by HE staining. The content of interleukin-18(IL-18) was observed by ELISA. The content of lactatedehydrogenase(LDH)was determined by microplate method. TUNEL assay kit was used to detect the cell death. The immunohistochemical staining was used to detect the expressions of NLRP3 and apoptosis-associated speck-like protein containing a CARD(ASC). Western blot was used to detect the expressions of interleukin-1β(IL-1β), caspase-1 and gasdermin D(GSDMD).The experimental study showed that compared with normal group, the LDH content, TUNEL positive staining, inflammatory factors(IL-18, IL-1β), and proteins associated with pyroptosis were significantly increased(P<0.05). Compared with model control group, the LDH content, TUNEL positive staining, inflammatory factors(IL-18, IL-1β), and proteins associated with pyroptosis were decreased, and these results were more significant in high-dose groups(P<0.05). The results of HE staining showed that Huangqin Decoction could improve the pathological changes of colon. Huangqin Decoction could inhibit UC cell pyroptosis, and the mechanism may be closely related to NLRP3/caspase-1 signaling pathway.
